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Structure paper

TitleStructural Basis of Nav1.7 Inhibition by a Gating-Modifier Spider Toxin.
Journal, issue, pagesCell, Vol. 176, Issue 4, Page 702-715.e14, Year 2019
Publish dateFeb 7, 2019
AuthorsHui Xu / Tianbo Li / Alexis Rohou / Christopher P Arthur / Foteini Tzakoniati / Evera Wong / Alberto Estevez / Christine Kugel / Yvonne Franke / Jun Chen / Claudio Ciferri / David H Hackos / Christopher M Koth / Jian Payandeh /
PubMed AbstractVoltage-gated sodium (Nav) channels are targets of disease mutations, toxins, and therapeutic drugs. Despite recent advances, the structural basis of voltage sensing, electromechanical coupling, and ...Voltage-gated sodium (Nav) channels are targets of disease mutations, toxins, and therapeutic drugs. Despite recent advances, the structural basis of voltage sensing, electromechanical coupling, and toxin modulation remains ill-defined. Protoxin-II (ProTx2) from the Peruvian green velvet tarantula is an inhibitor cystine-knot peptide and selective antagonist of the human Nav1.7 channel. Here, we visualize ProTx2 in complex with voltage-sensor domain II (VSD2) from Nav1.7 using X-ray crystallography and cryoelectron microscopy. Membrane partitioning orients ProTx2 for unfettered access to VSD2, where ProTx2 interrogates distinct features of the Nav1.7 receptor site. ProTx2 positions two basic residues into the extracellular vestibule to antagonize S4 gating-charge movement through an electrostatic mechanism. ProTx2 has trapped activated and deactivated states of VSD2, revealing a remarkable ∼10 Å translation of the S4 helix, providing a structural framework for activation gating in voltage-gated ion channels. Finally, our results deliver key templates to design selective Nav channel antagonists.
External linksCell / PubMed:30661758
MethodsEM (single particle) / X-ray diffraction
Resolution3.541 - 4.2 Å
Structure data

EMDB-0341, PDB-6n4q:
CryoEM structure of Nav1.7 VSD2 (actived state) in complex with the gating modifier toxin ProTx2
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-0342, PDB-6n4r:
CryoEM structure of Nav1.7 VSD2 (deactived state) in complex with the gating modifier toxin ProTx2
Method: EM (single particle) / Resolution: 4.2 Å

PDB-6n4i:
Structural basis of Nav1.7 inhibition by a gating-modifier spider toxin
Method: X-RAY DIFFRACTION / Resolution: 3.541 Å

Chemicals

ChemComp-6OU:
[(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate / phospholipid*YM

Source
  • homo sapiens (human)
  • arcobacter butzleri (strain rm4018) (bacteria)
  • thrixopelma pruriens (green velvet)
  • mouse (mice)
  • mus musculus (house mouse)
KeywordsMETAL TRANSPORT / sodium channel / toxin / gating-modifier / voltage-gated / MEMBRANE PROTEIN / voltage-gated sodium channel / gating modifier toxin

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