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Structure paper

TitleMechanistic Insights into the cis- and trans-Acting DNase Activities of Cas12a.
Journal, issue, pagesMol. Cell, Vol. 73, Page 589-600.e4, Year 2019
Publish dateOct 29, 2018 (structure data deposition date)
AuthorsSwarts, D.C. / Jinek, M.
External linksMol. Cell / PubMed:30639240
MethodsX-ray diffraction
Resolution2.65 - 2.98 Å
Structure data

PDB-6i1k:
Crystal structure of catalytically inactive FnCas12a in complex with a crRNA guide and a dsDNA target
Method: X-RAY DIFFRACTION / Resolution: 2.65 Å

PDB-6i1l:
Crystal structure of FnCas12a in complex with a crRNA guide and ssDNA target
Method: X-RAY DIFFRACTION / Resolution: 2.98 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-CIT:
CITRIC ACID

ChemComp-K:
Unknown entry

Source
  • francisella tularensis subsp. novicida (strain u112) (bacteria)
  • synthetic construct (others)
KeywordsHYDROLASE / CRISPR-Cas12a / FnCas12a / CRISPR-Cpf1 / FnCpf1

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