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Structure paper

TitleCryo-EM structure of the yeast respiratory supercomplex.
Journal, issue, pagesNat Struct Mol Biol, Vol. 26, Issue 1, Page 50-57, Year 2019
Publish dateDec 31, 2018
AuthorsSorbhi Rathore / Jens Berndtsson / Lorena Marin-Buera / Julian Conrad / Marta Carroni / Peter Brzezinski / Martin Ott /
PubMed AbstractRespiratory chain complexes execute energy conversion by connecting electron transport with proton translocation over the inner mitochondrial membrane to fuel ATP synthesis. Notably, these complexes ...Respiratory chain complexes execute energy conversion by connecting electron transport with proton translocation over the inner mitochondrial membrane to fuel ATP synthesis. Notably, these complexes form multi-enzyme assemblies known as respiratory supercomplexes. Here we used single-particle cryo-EM to determine the structures of the yeast mitochondrial respiratory supercomplexes IIIIV and IIIIV, at 3.2-Å and 3.5-Å resolutions, respectively. We revealed the overall architecture of the supercomplex, which deviates from the previously determined assemblies in mammals; obtained a near-atomic structure of the yeast complex IV; and identified the protein-protein and protein-lipid interactions implicated in supercomplex formation. Take together, our results demonstrate convergent evolution of supercomplexes in mitochondria that, while building similar assemblies, results in substantially different arrangements and structural solutions to support energy conversion.
External linksNat Struct Mol Biol / PubMed:30598556
MethodsEM (single particle)
Resolution3.23 - 3.5 Å
Structure data

0004

6giq

EMDB-0004, PDB-6giq:
Saccharomyces cerevisiae respiratory supercomplex III2IV
Method: EM (single particle) / Resolution: 3.23 Å

EMDB-0005:
Saccharomyces cerevisiae supercomplex class 1 (III2IV)
Method: EM (single particle) / Resolution: 3.34 Å

EMDB-0006:
Saccharomyces cerevisiae supercomplex class2 (III2IV2)
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-6PH:
(1R)-2-(phosphonooxy)-1-[(tridecanoyloxy)methyl]ethyl pentadecanoate / Phosphatidic acid

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

ChemComp-8PE:
(2R)-3-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(tetradecanoyloxy)propyl octadecanoate / phospholipid*YM

ChemComp-CN5:
(5S,11R)-5,8,11-trihydroxy-5,11-dioxido-17-oxo-4,6,10,12,16-pentaoxa-5,11-diphosphaoctadec-1-yl pentadecanoate / Cardiolipin

ChemComp-UQ6:
5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL

ChemComp-7PH:
(1R)-2-(dodecanoyloxy)-1-[(phosphonooxy)methyl]ethyl tetradecanoate / Phosphatidic acid

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

ChemComp-PCF:
1,2-DIACYL-SN-GLYCERO-3-PHOSHOCHOLINE / Dipalmitoylphosphatidylcholine

ChemComp-9PE:
(1R)-2-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(heptanoyloxy)methyl]ethyl octadecanoate / phospholipid*YM

ChemComp-CN3:
(2R,5S,11R,14R)-5,8,11-trihydroxy-2-(nonanoyloxy)-5,11-dioxido-16-oxo-14-[(propanoyloxy)methyl]-4,6,10,12,15-pentaoxa-5,11-diphosphanonadec-1-yl undecanoate / Cardiolipin

ChemComp-CU:
COPPER (II) ION / Copper

ChemComp-HEA:
HEME-A / Heme A

ChemComp-CUA:
DINUCLEAR COPPER ION

Source
  • saccharomyces cerevisiae w303 (yeast)
  • Baker's yeast (brewer's yeast)
KeywordsELECTRON TRANSPORT / Respiratory chain / supercomplex / bc1 complex / cytochrome c oxidase

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