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-Structure paper
| タイトル | Structure of yeast cytochrome c oxidase in a supercomplex with cytochrome bc. |
|---|---|
| ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 26, Issue 1, Page 78-83, Year 2019 |
| 掲載日 | 2018年12月31日 |
著者 | Andrew M Hartley / Natalya Lukoyanova / Yunyi Zhang / Alfredo Cabrera-Orefice / Susanne Arnold / Brigitte Meunier / Nikos Pinotsis / Amandine Maréchal / ![]() |
| PubMed 要旨 | Cytochrome c oxidase (complex IV, CIV) is known in mammals to exist independently or in association with other respiratory proteins to form supercomplexes (SCs). In Saccharomyces cerevisiae, CIV is ...Cytochrome c oxidase (complex IV, CIV) is known in mammals to exist independently or in association with other respiratory proteins to form supercomplexes (SCs). In Saccharomyces cerevisiae, CIV is found solely in an SC with cytochrome bc (complex III, CIII). Here, we present the cryogenic electron microscopy (cryo-EM) structure of S. cerevisiae CIV in a IIIIV SC at 3.3 Å resolution. While overall similarity to mammalian homologs is high, we found notable differences in the supernumerary subunits Cox26 and Cox13; the latter exhibits a unique arrangement that precludes CIV dimerization as seen in bovine. A conformational shift in the matrix domain of Cox5A-involved in allosteric inhibition by ATP-may arise from its association with CIII. The CIII-CIV arrangement highlights a conserved interaction interface of CIII, albeit one occupied by complex I in mammalian respirasomes. We discuss our findings in the context of the potential impact of SC formation on CIV regulation. |
リンク | Nat Struct Mol Biol / PubMed:30598554 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 3.31 - 3.38 Å |
| 構造データ | EMDB-0262, PDB-6hu9: ![]() EMDB-0268: ![]() EMDB-0269: |
| 化合物 | ![]() ChemComp-PEF: ![]() ChemComp-HEM: ![]() ChemComp-UQ6: ![]() ChemComp-CDL: ![]() ChemComp-PCF: ![]() ChemComp-HEC: ![]() ChemComp-FES: ![]() ChemComp-CU: ![]() ChemComp-HEA: ![]() ChemComp-CA: ![]() ChemComp-MG: ![]() ChemComp-CUA: ![]() ChemComp-ZN: |
| 由来 |
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キーワード | OXIDOREDUCTASE/ELECTRON TRANSPORT / Cytochrome c oxidase Cytochrome bc1 Mitochondria Respiratory chain Supercomplex / OXIDOREDUCTASE / ELECTRON TRANSPORT / OXIDOREDUCTASE-ELECTRON TRANSPORT complex |
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