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Structure paper

TitleStructure of yeast cytochrome c oxidase in a supercomplex with cytochrome bc.
Journal, issue, pagesNat Struct Mol Biol, Vol. 26, Issue 1, Page 78-83, Year 2019
Publish dateDec 31, 2018
AuthorsAndrew M Hartley / Natalya Lukoyanova / Yunyi Zhang / Alfredo Cabrera-Orefice / Susanne Arnold / Brigitte Meunier / Nikos Pinotsis / Amandine Maréchal /
PubMed AbstractCytochrome c oxidase (complex IV, CIV) is known in mammals to exist independently or in association with other respiratory proteins to form supercomplexes (SCs). In Saccharomyces cerevisiae, CIV is ...Cytochrome c oxidase (complex IV, CIV) is known in mammals to exist independently or in association with other respiratory proteins to form supercomplexes (SCs). In Saccharomyces cerevisiae, CIV is found solely in an SC with cytochrome bc (complex III, CIII). Here, we present the cryogenic electron microscopy (cryo-EM) structure of S. cerevisiae CIV in a IIIIV SC at 3.3 Å resolution. While overall similarity to mammalian homologs is high, we found notable differences in the supernumerary subunits Cox26 and Cox13; the latter exhibits a unique arrangement that precludes CIV dimerization as seen in bovine. A conformational shift in the matrix domain of Cox5A-involved in allosteric inhibition by ATP-may arise from its association with CIII. The CIII-CIV arrangement highlights a conserved interaction interface of CIII, albeit one occupied by complex I in mammalian respirasomes. We discuss our findings in the context of the potential impact of SC formation on CIV regulation.
External linksNat Struct Mol Biol / PubMed:30598554 / PubMed Central
MethodsEM (single particle)
Resolution3.31 - 3.38 Å
Structure data

0262

6hu9

EMDB-0262, PDB-6hu9:
III2-IV2 mitochondrial respiratory supercomplex from S. cerevisiae
Method: EM (single particle) / Resolution: 3.31 Å

EMDB-0268:
Complex IV in the III2-IV2 mitochondrial respiratory supercomplex from S. cerevisiae (CIVb)
Method: EM (single particle) / Resolution: 3.38 Å

EMDB-0269:
Complex IV in the III2-IV2 mitochondrial respiratory supercomplex from S. cerevisiae (CIVa)
Method: EM (single particle) / Resolution: 3.31 Å

Chemicals

ChemComp-PEF:
DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE / phospholipid*YM / Phosphatidylethanolamine

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

ChemComp-UQ6:
5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

ChemComp-PCF:
1,2-DIACYL-SN-GLYCERO-3-PHOSHOCHOLINE / Dipalmitoylphosphatidylcholine

ChemComp-HEC:
HEME C / Heme C

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

ChemComp-CU:
COPPER (II) ION / Copper

ChemComp-HEA:
HEME-A / Heme A

ChemComp-CA:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-CUA:
DINUCLEAR COPPER ION

ChemComp-ZN:
Unknown entry

Source
  • Saccharomyces cerevisiae (brewer's yeast)
  • Saccharomyces cerevisi (brewer's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
KeywordsOXIDOREDUCTASE/ELECTRON TRANSPORT / Cytochrome c oxidase Cytochrome bc1 Mitochondria Respiratory chain Supercomplex / OXIDOREDUCTASE / ELECTRON TRANSPORT / OXIDOREDUCTASE-ELECTRON TRANSPORT complex

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