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Structure paper

TitleA Small Helical Bundle Prepares Primer Synthesis by Binding Two Nucleotides that Enhance Sequence-Specific Recognition of the DNA Template.
Journal, issue, pagesCell, Vol. 176, Page 154-166.e13, Year 2019
Publish dateJun 15, 2018 (structure data deposition date)
AuthorsBoudet, J. / Devillier, J.C. / Wiegand, T. / Salmon, L. / Meier, B.H. / Lipps, G. / Allain, F.H.
External linksCell / PubMed:30595448
MethodsNMR (solution) / NMR (solid-state)
Structure data

PDB-6gt7:
NMR structure of the free helix bundle domain from the functional pRN1 primase
Method: SOLUTION NMR

PDB-6gvq:
DNA-bound pRN1 helix bundle domain with ATP and magnesium in the interaction buffer
Method: SOLUTION NMR

PDB-6gvt:
Hybrid structure of the pRN1 helix bundle domain in complex with DNA and 2 ATP molecules
Method: SOLUTION NMR / SOLID-STATE NMR

PDB-6gvu:
NMR structure of the DNA-bound helix bundle domain from the functional pRN1 primase
Method: SOLUTION NMR

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • sulfolobus islandicus (archaea)
KeywordsTRANSFERASE / functional pRN1 primase / replication initiation / dinucleotide formation / unusual archaeo-eukaryotic primase / DNA BINDING PROTEIN / pRN1 primase / quaternary structure of the helix bundle domain / synergistic effect / primase / single-stranded DNA

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