EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization.
ジャーナル・号・ページ	Cell Rep, Vol. 25, Issue 11, Page 3136-33147.e5, Year 2018
掲載日	2018年12月11日
著者	S Saif Hasan / Chengqun Sun / Arthur S Kim / Yasunori Watanabe / Chun-Liang Chen / Thomas Klose / Geeta Buda / Max Crispin / Michael S Diamond / William B Klimstra / Michael G Rossmann /
PubMed 要旨	Alphaviruses are enveloped pathogens that cause arthritis and encephalitis. Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an ...Alphaviruses are enveloped pathogens that cause arthritis and encephalitis. Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans. Our analysis provides insights into viral entry into host cells. The envelope protein E2 showed a binding site for the cellular attachment factor heparan sulfate. The presence of a cryptic E2 glycan suggests how EEEV escapes surveillance by lectin-expressing myeloid lineage cells, which are sentinels of the immune system. A mechanism for nucleocapsid core release and disassembly upon viral entry was inferred based on pH changes and capsid dissociation from envelope proteins. The EEEV capsid structure showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release. Using five Fab-EEEV complexes derived from neutralizing antibodies, our investigation provides insights into EEEV host cell interactions and protective epitopes relevant to vaccine design.
リンク	Cell Rep / PubMed:30540945 / PubMed Central
手法	EM (単粒子)
解像度	4.4 - 8.2 Å
構造データ	9249 6mui EMDB-9249, PDB-6mui: CryoEM structure of chimeric Eastern Equine Encephalitis Virus with Fab of EEEV-42 antibody 手法: EM (単粒子) / 解像度: 7.7 Å 9274 6mw9 EMDB-9274, PDB-6mw9: CryoEM structure of chimeric Eastern Equine Encephalitis Virus with Fab of EEEV-3 antibody 手法: EM (単粒子) / 解像度: 7.3 Å 9275 6mwc EMDB-9275, PDB-6mwc: CryoEM structure of chimeric Eastern Equine Encephalitis Virus with Fab of EEEV-5 antibody 手法: EM (単粒子) / 解像度: 7.5 Å 9278 6mwv EMDB-9278, PDB-6mwv: CryoEM structure of Chimeric Eastern Equine Encephalitis Virus with Fab of EEEV-58 Antibody 手法: EM (単粒子) / 解像度: 7.3 Å 9279 6mwx EMDB-9279, PDB-6mwx: CryoEM structure of Chimeric Eastern Equine Encephalitis Virus with Fab of EEEV-69 Antibody 手法: EM (単粒子) / 解像度: 8.2 Å 9280 6mx4 EMDB-9280, PDB-6mx4: CryoEM structure of chimeric Eastern Equine Encephalitis Virus 手法: EM (単粒子) / 解像度: 4.4 Å 9281 6mx7 EMDB-9281, PDB-6mx7: CryoEM structure of chimeric Eastern Equine Encephalitis Virus: Genome-Binding Capsid N-terminal Domain 手法: EM (単粒子) / 解像度: 4.8 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	mus musculus (ハツカネズミ) EEEV (東部ウマ脳炎ウイルス) Mouse (ネズミ) eastern equine encephalitis virus (東部ウマ脳炎ウイルス)
キーワード	VIRUS/IMMUNE SYSTEM / Alphavirus / EEEV / Eastern Equine Encephalitis Virus / Sindbis / Fab / VIRUS-IMMUNE SYSTEM complex / VIRUS