Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure Studies of the CRISPR-Csm Complex Reveal Mechanism of Co-transcriptional Interference.
Journal, issue, pages	Cell, Vol. 176, Issue 1-2, Page 239-253.e16, Year 2019
Publish date	Jan 10, 2019
Authors	Lilan You / Jun Ma / Jiuyu Wang / Daria Artamonova / Min Wang / Liang Liu / Hua Xiang / Konstantin Severinov / Xinzheng Zhang / Yanli Wang /
PubMed Abstract	Csm, a type III-A CRISPR-Cas interference complex, is a CRISPR RNA (crRNA)-guided RNase that also possesses target RNA-dependent DNase and cyclic oligoadenylate (cOA) synthetase activities. However, ...Csm, a type III-A CRISPR-Cas interference complex, is a CRISPR RNA (crRNA)-guided RNase that also possesses target RNA-dependent DNase and cyclic oligoadenylate (cOA) synthetase activities. However, the structural features allowing target RNA-binding-dependent activation of DNA cleavage and cOA generation remain unknown. Here, we report the structure of Csm in complex with crRNA together with structures of cognate or non-cognate target RNA bound Csm complexes. We show that depending on complementarity with the 5' tag of crRNA, the 3' anti-tag region of target RNA binds at two distinct sites of the Csm complex. Importantly, the interaction between the non-complementary anti-tag region of cognate target RNA and Csm1 induces a conformational change at the Csm1 subunit that allosterically activates DNA cleavage and cOA generation. Together, our structural studies provide crucial insights into the mechanistic processes required for crRNA-meditated sequence-specific RNA cleavage, RNA target-dependent non-specific DNA cleavage, and cOA generation.
External links	Cell / PubMed:30503210 / PubMed Central
Methods	EM (single particle) / X-ray diffraction
Resolution	2.9 - 3.8 Å
Structure data	9653 6ifk EMDB-9653, PDB-6ifk: Cryo-EM structure of type III-A Csm-CTR1 complex, AMPPNP bound Method: EM (single particle) / Resolution: 3.2 Å 9654 6ifl EMDB-9654, PDB-6ifl: Cryo-EM structure of type III-A Csm-NTR complex Method: EM (single particle) / Resolution: 3.16 Å EMDB-9655: Cryo-EM structure of type III-A Csm complex Method: EM (single particle) / Resolution: 3.8 Å 9656 6ifr EMDB-9656, PDB-6ifr: Type III-A Csm complex, Cryo-EM structure of Csm-NTR, ATP bound Method: EM (single particle) / Resolution: 3.4 Å 9657 6ifu EMDB-9657, PDB-6ifu: Cryo-EM structure of type III-A Csm-CTR2-dsDNA complex Method: EM (single particle) / Resolution: 3.05 Å 9658 6ify EMDB-9658, PDB-6ify: Type III-A Csm complex, Cryo-EM structure of Csm-CTR1 Method: EM (single particle) / Resolution: 3.8 Å 9659 6ifz EMDB-9659, PDB-6ifz: Type III-A Csm complex, Cryo-EM structure of Csm-CTR2-ssDNA complex Method: EM (single particle) / Resolution: 3.58 Å 9660 6ig0 EMDB-9660, PDB-6ig0: Type III-A Csm complex, Cryo-EM structure of Csm-CTR1, ATP bound Method: EM (single particle) / Resolution: 3.37 Å PDB-6ifn: Crystal structure of Type III-A CRISPR Csm complex Method: X-RAY DIFFRACTION / Resolution: 2.9 Å
Chemicals	ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogueYM ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry ChemComp-MN: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM / Adenosine triphosphate
Source	streptococcus thermophilus nd03 (bacteria) streptococcus thermophilus (bacteria)
Keywords	RNA BINDING PROTEIN / Csm complex / Type III-A / CRISPR-Cas system