Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	The Core and Holoenzyme Forms of RNA Polymerase from .
Journal, issue, pages	J Bacteriol, Vol. 201, Issue 4, Year 2019
Publish date	Feb 15, 2019
Authors	Tomáš Kouba / Jiří Pospíšil / Jarmila Hnilicová / Hana Šanderová / Ivan Barvík / Libor Krásný /
PubMed Abstract	Bacterial RNA polymerase (RNAP) is essential for gene expression and as such is a valid drug target. Hence, it is imperative to know its structure and dynamics. Here, we present two as-yet-unreported ...Bacterial RNA polymerase (RNAP) is essential for gene expression and as such is a valid drug target. Hence, it is imperative to know its structure and dynamics. Here, we present two as-yet-unreported forms of RNAP: core and holoenzyme containing σ but no other factors. Each form was detected by cryo-electron microscopy in two major conformations. Comparisons of these structures with known structures of other RNAPs reveal a high degree of conformational flexibility of the mycobacterial enzyme and confirm that region 1.1 of σ is directed into the primary channel of RNAP. Taken together, we describe the conformational changes of unrestrained mycobacterial RNAP. We describe here three-dimensional structures of core and holoenzyme forms of mycobacterial RNA polymerase (RNAP) solved by cryo-electron microscopy. These structures fill the thus-far-empty spots in the gallery of the pivotal forms of mycobacterial RNAP and illuminate the extent of conformational dynamics of this enzyme. The presented findings may facilitate future designs of antimycobacterial drugs targeting RNAP.
External links	J Bacteriol / PubMed:30478083 / PubMed Central
Methods	EM (single particle)
Resolution	3.81 - 4.22 Å
Structure data	3983 6eyd EMDB-3983, PDB-6eyd: Structure of Mycobacterium smegmatis RNA polymerase Sigma-A holoenzyme Method: EM (single particle) / Resolution: 4.22 Å 4192 6f6w EMDB-4192, PDB-6f6w: Structure of Mycobacterium smegmatis RNA polymerase core Method: EM (single particle) / Resolution: 3.81 Å
Chemicals	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry
Source	Mycobacterium smegmatis str. MC2 155 (bacteria) mycobacterium smegmatis (strain atcc 700084 / mc(2)155) (bacteria) mycolicibacterium smegmatis mc2 155 (bacteria)
Keywords	TRANSCRIPTION / TRANSCRIPTION Sigma