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Structure paper

TitleStructural basis for recognition and repair of the 3'-phosphate by NExo, a base excision DNA repair nuclease from Neisseria meningitidis.
Journal, issue, pagesNucleic Acids Res., Vol. 46, Page 11980-11989, Year 2018
Publish dateJan 23, 2018 (structure data deposition date)
AuthorsSilhan, J. / Zhao, Q. / Boura, E. / Thomson, H. / Forster, A. / Tang, C.M. / Freemont, P.S. / Baldwin, G.S.
External linksNucleic Acids Res. / PubMed:30329088
MethodsX-ray diffraction
Resolution2.02 - 2.297 Å
Structure data

PDB-6fk4:
Structure of 3' phosphatase NExo (WT) from Neisseria bound to DNA substrate
Method: X-RAY DIFFRACTION / Resolution: 2.297 Å

PDB-6fk5:
Structure of 3' phosphatase NExo (D146N) from Neisseria bound to DNA substrate in presence of magnesium ion
Method: X-RAY DIFFRACTION / Resolution: 2.02 Å

PDB-6fke:
Structure of 3' phosphatase NExo (D146N) from Neisseria bound to product DNA hairpin
Method: X-RAY DIFFRACTION / Resolution: 2.151 Å

Chemicals

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-MN:
Unknown entry

Source
  • neisseria meningitidis serogroup b (strain mc58) (bacteria)
  • other sequences (unknown)
  • neisseria meningitidis mc58 (bacteria)
  • synthetic construct (others)
KeywordsDNA BINDING PROTEIN / 3' phosphatase / base excision DNA repair. / base excision DNA repair / Mg2+

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