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TitleSuppressor mutations in Rpf2-Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation.
Journal, issue, pagesNat Commun, Vol. 9, Issue 1, Page 4094, Year 2018
Publish dateOct 5, 2018
AuthorsMatthias Thoms / Valentin Mitterer / Lukas Kater / Laurent Falquet / Roland Beckmann / Dieter Kressler / Ed Hurt /
PubMed AbstractDuring eukaryotic 60S biogenesis, the 5S RNP requires a large rotational movement to achieve its mature position. Cryo-EM of the Rix1-Rea1 pre-60S particle has revealed the post-rotation stage, in ...During eukaryotic 60S biogenesis, the 5S RNP requires a large rotational movement to achieve its mature position. Cryo-EM of the Rix1-Rea1 pre-60S particle has revealed the post-rotation stage, in which a gently undulating α-helix corresponding to Cgr1 becomes wedged between Rsa4 and the relocated 5S RNP, but the purpose of this insertion was unknown. Here, we show that cgr1 deletion in yeast causes a slow-growth phenotype and reversion of the pre-60S particle to the pre-rotation stage. However, spontaneous extragenic suppressors could be isolated, which restore growth and pre-60S biogenesis in the absence of Cgr1. Whole-genome sequencing reveals that the suppressor mutations map in the Rpf2-Rrs1 module and Rpl5, which together stabilize the unrotated stage of the 5S RNP. Thus, mutations in factors stabilizing the pre-rotation stage facilitate 5S RNP relocation upon deletion of Cgr1, but Cgr1 itself could stabilize the post-rotation stage.
External linksNat Commun / PubMed:30291245 / PubMed Central
MethodsEM (single particle)
Resolution11.0 - 16.0 Å
Structure data

EMDB-0218:
Cryo-EM structure 1 of pre-60S particles isolated via Arx1-FTpA from yeast
Method: EM (single particle) / Resolution: 11.0 Å

EMDB-0219:
Cryo-EM structure 2 of pre-60S particles isolated via Arx1-FTpA from yeast
Method: EM (single particle) / Resolution: 16.0 Å

EMDB-0220:
Cryo-EM structure 3 of pre-60S particles isolated via Arx1-FTpA from yeast
Method: EM (single particle) / Resolution: 12.0 Å

EMDB-0221:
Cryo-EM structure of a pre-60S particle isolated via Arx1-FTpA from a cgr1 depleted yeast strain
Method: EM (single particle) / Resolution: 12.0 Å

EMDB-0222:
Cryo-EM structure 1 of pre-60S particles isolated via Arx1-FTpA from a cgr1 depleted yeast strain in the presence of the rrs1E102D suppressor
Method: EM (single particle) / Resolution: 14.0 Å

EMDB-0223:
Cryo-EM structure 2 of pre-60S particles isolated via Arx1-FTpA from a cgr1 depleted yeast strain in the presence of the rrs1E102D suppressor
Method: EM (single particle) / Resolution: 14.0 Å

EMDB-0224:
Cryo-EM structure 3 of pre-60S particles isolated via Arx1-FTpA from a cgr1 depleted yeast strain in the presence of the rrs1E102D suppressor
Method: EM (single particle) / Resolution: 14.0 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)

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