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TitleMetal-free class Ie ribonucleotide reductase from pathogens initiates catalysis with a tyrosine-derived dihydroxyphenylalanine radical.
Journal, issue, pagesProc. Natl. Acad. Sci. U.S.A., Vol. 115, Page 10022-10027, Year 2018
Publish dateAug 6, 2018 (structure data deposition date)
AuthorsBlaesi, E.J. / Palowitch, G.M. / Hu, K. / Kim, A.J. / Rose, H.R. / Alapati, R. / Lougee, M.G. / Kim, H.J. / Taguchi, A.T. / Tan, K.O. ...Blaesi, E.J. / Palowitch, G.M. / Hu, K. / Kim, A.J. / Rose, H.R. / Alapati, R. / Lougee, M.G. / Kim, H.J. / Taguchi, A.T. / Tan, K.O. / Laremore, T.N. / Griffin, R.G. / Krebs, C. / Matthews, M.L. / Silakov, A. / Bollinger Jr., J.M. / Allen, B.D. / Boal, A.K.
External linksProc. Natl. Acad. Sci. U.S.A. / PubMed:30224458
MethodsX-ray diffraction
Resolution1.58 - 1.95 Å
Structure data

PDB-6ebo:
Crystal Structure of the Class Ie Ribonucleotide Reductase Beta Subunit from Aerococcus urinae in Unactivated Form
Method: X-RAY DIFFRACTION / Resolution: 1.58 Å

PDB-6ebp:
Crystal Structure of the Class Ie Ribonucleotide Reductase Beta Subunit from Aerococcus urinae in Activated Form
Method: X-RAY DIFFRACTION / Resolution: 1.59 Å

PDB-6ebq:
Crystal Structure of the Flavoprotein NrdI from Aerococcus urinae in Oxidized Form
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-6ebz:
Crystal Structure of the Class Ie Ribonucleotide Reductase Beta Subunit from Aerococcus urinae in Activated Form with Thiocyanate Bound
Method: X-RAY DIFFRACTION / Resolution: 1.66 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-SCN:
THIOCYANATE ION

Source
  • aerococcus urinae (strain acs-120-v-col10a) (bacteria)
  • aerococcus urinae (bacteria)
KeywordsOXIDOREDUCTASE / ribonucleotide reductase / beta subunit / RNR / R2 / class Ie / metal-free / post-translational modification / PTM / DOPA / radical / FLAVOPROTEIN / NrdI / FMN / SCN / thiocyanate

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