EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	High-resolution structures of HIV-1 Gag cleavage mutants determine structural switch for virus maturation.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 115, Issue 40, Page E9401-E9410, Year 2018
掲載日	2018年10月2日
著者	Simone Mattei / Aaron Tan / Bärbel Glass / Barbara Müller / Hans-Georg Kräusslich / John A G Briggs /
PubMed 要旨	HIV-1 maturation occurs via multiple proteolytic cleavages of the Gag polyprotein, causing rearrangement of the virus particle required for infectivity. Cleavage results in beta-hairpin formation at ...HIV-1 maturation occurs via multiple proteolytic cleavages of the Gag polyprotein, causing rearrangement of the virus particle required for infectivity. Cleavage results in beta-hairpin formation at the N terminus of the CA (capsid) protein and loss of a six-helix bundle formed by the C terminus of CA and the neighboring SP1 peptide. How individual cleavages contribute to changes in protein structure and interactions, and how the mature, conical capsid forms, are poorly understood. Here, we employed cryoelectron tomography to determine morphology and high-resolution CA lattice structures for HIV-1 derivatives in which Gag cleavage sites are mutated. These analyses prompt us to revise current models for the crucial maturation switch. Unlike previously proposed, cleavage on either terminus of CA was sufficient, in principle, for lattice maturation, while complete processing was needed for conical capsid formation. We conclude that destabilization of the six-helix bundle, rather than beta-hairpin formation, represents the main determinant of structural maturation.
リンク	Proc Natl Acad Sci U S A / PubMed:30217893 / PubMed Central
手法	EM (サブトモグラム平均) / EM (トモグラフィー)
解像度	3.7 - 24.0 Å
構造データ	EMDB-0164: Immature CA domain from HIV-1 MA-SP1 Gag proteolytic cleavage mutant virus particles 手法: EM (サブトモグラム平均) / 解像度: 4.0 Å EMDB-0165: Immature CA domain from HIV-1 MA-CA Gag proteolytic cleavage mutant virus particles 手法: EM (サブトモグラム平均) / 解像度: 3.7 Å EMDB-0166: Immature CA domain from HIV-1 CA-NC Gag proteolytic cleavage mutant virus particles 手法: EM (サブトモグラム平均) / 解像度: 4.5 Å EMDB-0167: Mature CA domain hexamer from HIV-1 MA-CA Gag proteolytic cleavage mutant virus particles 手法: EM (サブトモグラム平均) / 解像度: 8.3 Å EMDB-0168: Mature CA domain hexamer from HIV-1 CA-NC Gag proteolytic cleavage mutant virus particles 手法: EM (サブトモグラム平均) / 解像度: 9.7 Å EMDB-0169: Mature CA domain hexamer from HIV-1 CA-SP1 Gag proteolytic cleavage mutant virus particles 手法: EM (サブトモグラム平均) / 解像度: 7.9 Å EMDB-0170: Mature CA domain pentamer from HIV-1 CA-NC Gag proteolytic cleavage mutant virus particles 手法: EM (サブトモグラム平均) / 解像度: 22.0 Å EMDB-0171: Mature CA domain pentamer from HIV-1 CA-SP1 Gag proteolytic cleavage mutant virus particles 手法: EM (サブトモグラム平均) / 解像度: 24.0 Å EMDB-13079: Representative cryo-electron tomogram of HIV-1 MA-SP1 particles 手法: EM (トモグラフィー) EMDB-13080: Representative cryo-electron tomogram of HIV-1 MA-CA particles 手法: EM (トモグラフィー)
由来	Homo sapiens (ヒト)