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Structure paper

TitleAminoglycoside ribosome interactions reveal novel conformational states at ambient temperature.
Journal, issue, pagesNucleic Acids Res., Vol. 46, Page 9793-9804, Year 2018
Publish dateJan 31, 2018 (structure data deposition date)
AuthorsO'Sullivan, M.E. / Poitevin, F. / Sierra, R.G. / Gati, C. / Dao, E.H. / Rao, Y. / Aksit, F. / Ciftci, H. / Corsepius, N. / Greenhouse, R. ...O'Sullivan, M.E. / Poitevin, F. / Sierra, R.G. / Gati, C. / Dao, E.H. / Rao, Y. / Aksit, F. / Ciftci, H. / Corsepius, N. / Greenhouse, R. / Hayes, B. / Hunter, M.S. / Liang, M. / McGurk, A. / Mbgam, P. / Obrinsky, T. / Pardo-Avila, F. / Seaberg, M.H. / Cheng, A.G. / Ricci, A.J. / DeMirci, H.
External linksNucleic Acids Res. / PubMed:30113694
MethodsX-ray diffraction
Resolution3.4 - 3.5 Å
Structure data

PDB-6car:
Serial Femtosecond X-ray Crystal Structure of 30S ribosomal subunit from Thermus thermophilus in complex with Sisomicin
Method: X-RAY DIFFRACTION / Resolution: 3.4 Å

PDB-6cas:
Serial Femtosecond X-ray Crystal Structure of 30S ribosomal subunit from Thermus thermophilus in complex with N1MS
Method: X-RAY DIFFRACTION / Resolution: 3.5 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-SIS:
(1S,2S,3R,4S,6R)-4,6-diamino-3-{[(2S,3R)-3-amino-6-(aminomethyl)-3,4-dihydro-2H-pyran-2-yl]oxy}-2-hydroxycyclohexyl 3-deoxy-4-C-methyl-3-(methylamino)-beta-L-arabinopyranoside / antibiotic*YM

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-EUS:
N-[(1R,2S,3S,4R,5S)-5-amino-4-{[(2S,3R)-3-amino-6-(aminomethyl)-3,4-dihydro-2H-pyran-2-yl]oxy}-2-{[3-deoxy-4-C-methyl-3-(methylamino)-beta-L-arabinopyranosyl]oxy}-3-hydroxycyclohexyl]methanesulfonamide

Source
  • thermus thermophilus hb8 (bacteria)
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
KeywordsRIBOSOME / translation / miscoding / antibiotic / sisomicin / N1MS

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