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Structure paper

TitleStructural basis for the synergy of 4'- and 2'-modifications on siRNA nuclease resistance, thermal stability and RNAi activity.
Journal, issue, pagesNucleic Acids Res., Vol. 46, Page 8090-8104, Year 2018
Publish dateApr 4, 2018 (structure data deposition date)
AuthorsHarp, J.M. / Guenther, D.C. / Bisbe, A. / Perkins, L. / Matsuda, S. / Bommineni, G.R. / Zlatev, I. / Foster, D.J. / Taneja, N. / Charisse, K. ...Harp, J.M. / Guenther, D.C. / Bisbe, A. / Perkins, L. / Matsuda, S. / Bommineni, G.R. / Zlatev, I. / Foster, D.J. / Taneja, N. / Charisse, K. / Maier, M.A. / Rajeev, K.G. / Manoharan, M. / Egli, M.
External linksNucleic Acids Res. / PubMed:30107495
MethodsX-ray diffraction
Resolution1.4 - 2.398 Å
Structure data

PDB-6cxz:
RNA octamer containing 2'-F, 4'-Calpha-Me U.
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-6cy0:
RNA octamer containing 2'-F, 4'-Cbeta-OMe U.
Method: X-RAY DIFFRACTION / Resolution: 2.398 Å

PDB-6cy2:
RNA octamer containing 2'-OMe, 4'Calpha-OMe U.
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-6cy4:
RNA octamer containing 2'-OMe, 4'- Cbeta-OMe U.
Method: X-RAY DIFFRACTION / Resolution: 1.851 Å

Chemicals

ChemComp-CAC:
CACODYLATE ION

ChemComp-NCO:
COBALT HEXAMMINE(III)

ChemComp-HOH:
WATER

Source
  • synthetic construct (others)
KeywordsRNA / oligonucleotide / modified base

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