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TitleControllable Assembly of Flexible Protein Nanotubes for Loading Multifunctional Modules.
Journal, issue, pagesACS Appl Mater Interfaces, Vol. 10, Issue 30, Page 25135-25145, Year 2018
Publish dateAug 1, 2018
AuthorsGuibo Rao / Yan Fu / Na Li / Jiayi Yin / Jie Zhang / Manli Wang / Zhihong Hu / Sheng Cao /
PubMed AbstractViruses with filamentous morphologies, such as tobacco mosaic virus (TMV) and M13 bacteriophage, have long been studied as multivalent nanoscaffolds for loading functional motifs. Structural assembly ...Viruses with filamentous morphologies, such as tobacco mosaic virus (TMV) and M13 bacteriophage, have long been studied as multivalent nanoscaffolds for loading functional motifs. Structural assembly of the capsid proteins (CPs) of filamentous viruses often requires the presence of DNA or RNA molecules, which has limited their applications. Here, we describe a strategy for controllable assembly of flexible bio-nanotubes consisting of Escherichia coli expressed CP of baculovirus Helicoverpa armigera nucleopolyhedrovirus (HearNPV) in vitro. These protein-only nanotubes were studied as a new structural platform for high-density presentation of multiple active molecules on the exterior surface by direct fusion of the protein of interest to the N-terminus of HearNPV CP (HaCP). Structural characterization using cryoelectron microscopy demonstrated that the HaCP could assemble into two closely related but structurally distinct tube types, suggesting the tunable HaCP interaction network is the major contributor to the flexibility of HaCP nanotubes. Our flexible nanotubes could tolerate larger molecular modifications compared with TMV-based templates and could be used as promising candidates for versatile molecular loading applications.
External linksACS Appl Mater Interfaces / PubMed:29989404
MethodsEM (helical sym.)
Resolution14.0 - 21.0 Å
Structure data

EMDB-6933:
Cryo-EM reconstruction of flexible nanotubes self-assembled from baculovirus major capsid protein
Method: EM (helical sym.) / Resolution: 14.0 Å

EMDB-6936:
Cryo-EM reconstruction of flexible nanotubes self-assembled from baculovirus major capsid protein
Method: EM (helical sym.) / Resolution: 21.0 Å

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