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| Title | Natural chromatin is heterogeneous and self-associates in vitro. |
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| Journal, issue, pages | Mol Biol Cell, Vol. 29, Issue 13, Page 1652-1663, Year 2018 |
| Publish date | Jul 1, 2018 |
 Authors | Shujun Cai / Yajiao Song / Chen Chen / Jian Shi / Lu Gan /  |
| PubMed Abstract | The 30-nm fiber is commonly formed by oligonucleosome arrays in vitro but rarely found inside cells. To determine how chromatin higher-order structure is controlled, we used electron cryotomography ...The 30-nm fiber is commonly formed by oligonucleosome arrays in vitro but rarely found inside cells. To determine how chromatin higher-order structure is controlled, we used electron cryotomography (cryo-ET) to study the undigested natural chromatin released from two single-celled organisms in which 30-nm fibers have not been observed in vivo: picoplankton and yeast. In the presence of divalent cations, most of the chromatin from both organisms is condensed into a large mass in vitro. Rare irregular 30-nm fibers, some of which include face-to-face nucleosome interactions, do form at the periphery of this mass. In the absence of divalent cations, picoplankton chromatin decondenses into open zigzags. By contrast, yeast chromatin mostly remains condensed, with very few open motifs. Yeast chromatin packing is largely unchanged in the absence of linker histone and mildly decondensed when histones are more acetylated. Natural chromatin is therefore generally nonpermissive of regular motifs, even at the level of oligonucleosomes. |
 External links | Mol Biol Cell / PubMed:29742050 / PubMed Central |
| Methods | EM (tomography) |
| Structure data |  EMDB-6737: |
| Source |
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Ostreococcus tauri (plant)