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TitleStructure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface.
Journal, issue, pagesScience, Vol. 360, Issue 6388, Page 552-558, Year 2018
Publish dateMay 4, 2018
AuthorsSimon Jenni / Stephen C Harrison /
PubMed AbstractKinetochores connect mitotic-spindle microtubules with chromosomes, allowing microtubule depolymerization to pull chromosomes apart during anaphase while resisting detachment as the microtubule ...Kinetochores connect mitotic-spindle microtubules with chromosomes, allowing microtubule depolymerization to pull chromosomes apart during anaphase while resisting detachment as the microtubule shortens. The heterodecameric DASH/Dam1 complex (DASH/Dam1c), an essential component of yeast kinetochores, assembles into a microtubule-encircling ring. The ring associates with rodlike Ndc80 complexes to organize the kinetochore-microtubule interface. We report the cryo-electron microscopy structure (at ~4.5-angstrom resolution) of a DASH/Dam1c ring and a molecular model of its ordered components, validated by evolutionary direct-coupling analysis. Integrating this structure with that of the Ndc80 complex and with published interaction data yields a molecular picture of kinetochore-microtubule attachment, including how flexible, C-terminal extensions of DASH/Dam1c subunits project and contact widely separated sites on the Ndc80 complex rod and how phosphorylation at previously identified sites might regulate kinetochore assembly.
External linksScience / PubMed:29724956 / PubMed Central
MethodsEM (single particle)
Resolution4.5 - 6.5 Å
Structure data

EMDB-7446:
Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface (symmetric reconstruction)
Method: EM (single particle) / Resolution: 6.5 Å

7469

6cfz

EMDB-7469: Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface (asymmetric reconstruction)
PDB-6cfz: Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface
Method: EM (single particle) / Resolution: 4.5 Å

Source
  • chaetomium thermophilum (fungus)
KeywordsNUCLEAR PROTEIN / DASH / Dam1 complex / Ask1 / Dad1 / Dad2 / Dad3 / Dad4 / Dam1 / Duo1 / Hsk3 / Spc19 / Spc34 / kinetochore / kinetochore-microtubule interface / chromosome segregation / cell-division / point-centromere yeast

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