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- EMDB-7446: Structure of the DASH/Dam1 complex shows its role at the yeast ki... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-7446 | |||||||||
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Title | Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface (symmetric reconstruction) | |||||||||
![]() | DASH/Dam1c symmetric reconstruction | |||||||||
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Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 6.5 Å | |||||||||
![]() | Jenni S / Harrison SC | |||||||||
![]() | ![]() Title: Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface. Authors: Simon Jenni / Stephen C Harrison / ![]() Abstract: Kinetochores connect mitotic-spindle microtubules with chromosomes, allowing microtubule depolymerization to pull chromosomes apart during anaphase while resisting detachment as the microtubule ...Kinetochores connect mitotic-spindle microtubules with chromosomes, allowing microtubule depolymerization to pull chromosomes apart during anaphase while resisting detachment as the microtubule shortens. The heterodecameric DASH/Dam1 complex (DASH/Dam1c), an essential component of yeast kinetochores, assembles into a microtubule-encircling ring. The ring associates with rodlike Ndc80 complexes to organize the kinetochore-microtubule interface. We report the cryo-electron microscopy structure (at ~4.5-angstrom resolution) of a DASH/Dam1c ring and a molecular model of its ordered components, validated by evolutionary direct-coupling analysis. Integrating this structure with that of the Ndc80 complex and with published interaction data yields a molecular picture of kinetochore-microtubule attachment, including how flexible, C-terminal extensions of DASH/Dam1c subunits project and contact widely separated sites on the Ndc80 complex rod and how phosphorylation at previously identified sites might regulate kinetochore assembly. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 464.9 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 24 KB 24 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 21 KB | Display | ![]() |
Images | ![]() | 156.8 KB | ||
Masks | ![]() | 512 MB | ![]() | |
Others | ![]() ![]() | 154.4 MB 154.4 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 78.3 KB | Display | ![]() |
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Full document | ![]() | 77.5 KB | Display | |
Data in XML | ![]() | 494 B | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | DASH/Dam1c symmetric reconstruction | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.64 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
File | ![]() | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: Half-map 1 (not masked, not filtered)
File | emd_7446_half_map_1.map | ||||||||||||
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Annotation | Half-map 1 (not masked, not filtered) | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half-map 2 (not masked, not filtered)
File | emd_7446_half_map_2.map | ||||||||||||
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Annotation | Half-map 2 (not masked, not filtered) | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
+Entire : DASH/Dam1 complex
+Supramolecule #1: DASH/Dam1 complex
+Macromolecule #1: Ask1
+Macromolecule #2: Dad1
+Macromolecule #3: Dad2
+Macromolecule #4: Dad3
+Macromolecule #5: Dad4
+Macromolecule #6: Dam1
+Macromolecule #7: Duo1
+Macromolecule #8: Hsk3
+Macromolecule #9: Spc19
+Macromolecule #10: Spc34
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | helical array |
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Sample preparation
Concentration | 2.6 mg/mL |
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Buffer | pH: 7.5 |
Grid | Model: C-flat-1.2/1.3 4C / Material: COPPER / Mesh: 400 / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 89 % / Chamber temperature: 293 K / Instrument: GATAN CRYOPLUNGE 3 |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Number grids imaged: 1 / Number real images: 2723 / Average electron dose: 1.115 e/Å2 Details: Movies collected: 50 frames with 1.115 e/A2/per frame |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Calibrated magnification: 30488 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.4 µm / Nominal defocus min: 1.2 µm |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |