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Structure paper

TitleSubstrate-bound structures of a ketoreductase from amphotericin modular polyketide synthase.
Journal, issue, pagesJ. Struct. Biol., Vol. 203, Page 135-141, Year 2018
Publish dateJun 30, 2017 (structure data deposition date)
AuthorsLiu, C. / Yuan, M. / Xu, X. / Wang, L. / Keatinge-Clay, A.T. / Deng, Z. / Lin, S. / Zheng, J.
External linksJ. Struct. Biol. / PubMed:29626512
MethodsX-ray diffraction
Resolution1.8 - 1.96 Å
Structure data

PDB-5xwv:
Substrate-bound Structure of a Ketoreductase from the Second Module of the amphotericin Polyketide Synthases
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-5xww:
Substrate-bound Structure of G355T/Q364H mutant of a Ketoreductase from amphotericin Polyketide Synthases
Method: X-RAY DIFFRACTION / Resolution: 1.96 Å

Chemicals

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-8H6:
S-[2-[3-[[(2R)-3,3-dimethyl-2,4-bis(oxidanyl)butanoyl]amino]propanoylamino]ethyl] (2R)-2-methyl-3-oxidanylidene-pentanethioate

ChemComp-HOH:
WATER

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Source
  • streptomyces nodosus (bacteria)
KeywordsOXIDOREDUCTASE / modular polyketide synthease / ketoreductase / mutant

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