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-Structure paper
タイトル | Cryo-EM structure of the Blastochloris viridis LH1-RC complex at 2.9 Å. |
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ジャーナル・号・ページ | Nature, Vol. 556, Issue 7700, Page 203-208, Year 2018 |
掲載日 | 2018年4月4日 |
著者 | Pu Qian / C Alistair Siebert / Peiyi Wang / Daniel P Canniffe / C Neil Hunter / |
PubMed 要旨 | The light-harvesting 1-reaction centre (LH1-RC) complex is a key functional component of bacterial photosynthesis. Here we present a 2.9 Å resolution cryo-electron microscopy structure of the ...The light-harvesting 1-reaction centre (LH1-RC) complex is a key functional component of bacterial photosynthesis. Here we present a 2.9 Å resolution cryo-electron microscopy structure of the bacteriochlorophyll b-based LH1-RC complex from Blastochloris viridis that reveals the structural basis for absorption of infrared light and the molecular mechanism of quinone migration across the LH1 complex. The triple-ring LH1 complex comprises a circular array of 17 β-polypeptides sandwiched between 17 α- and 16 γ-polypeptides. Tight packing of the γ-apoproteins between β-polypeptides collectively interlocks and stabilizes the LH1 structure; this, together with the short Mg-Mg distances of bacteriochlorophyll b pairs, contributes to the large redshift of bacteriochlorophyll b absorption. The 'missing' 17th γ-polypeptide creates a pore in the LH1 ring, and an adjacent binding pocket provides a folding template for a quinone, Q , which adopts a compact, export-ready conformation before passage through the pore and eventual diffusion to the cytochrome bc complex. |
リンク | Nature / PubMed:29618818 |
手法 | EM (単粒子) |
解像度 | 2.87 Å |
構造データ | |
化合物 | ChemComp-HEM: ChemComp-BCB: ChemComp-BPB: ChemComp-UQ9: ChemComp-FE: ChemComp-SO4: ChemComp-LDA: ChemComp-MQ9: ChemComp-NS5: ChemComp-NS0: |
由来 |
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キーワード | PHOTOSYNTHESIS / Reaction centre light harvesting complex 1 Blc. viridis Cryo-EM RC-LH1 Photosynthesis |