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Structure paper

TitleYeast surface display platform for rapid discovery of conformationally selective nanobodies.
Journal, issue, pagesNat. Struct. Mol. Biol., Vol. 25, Page 289-296, Year 2018
Publish dateMay 1, 2017 (structure data deposition date)
AuthorsMcMahon, C. / Baier, A.S. / Pascolutti, R. / Wegrecki, M. / Zheng, S. / Ong, J.X. / Erlandson, S.C. / Hilger, D. / Rasmussen, S.G.F. / Ring, A.M. ...McMahon, C. / Baier, A.S. / Pascolutti, R. / Wegrecki, M. / Zheng, S. / Ong, J.X. / Erlandson, S.C. / Hilger, D. / Rasmussen, S.G.F. / Ring, A.M. / Manglik, A. / Kruse, A.C.
External linksNat. Struct. Mol. Biol. / PubMed:29434346
MethodsX-ray diffraction
Resolution1.4 - 2.6 Å
Structure data

PDB-5vnv:
Crystal structure of Nb.b201
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-5vnw:
Crystal structure of Nb.b201 bound to human serum albumin
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-K:
Unknown entry

ChemComp-FMT:
FORMIC ACID

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

ChemComp-PLM:
PALMITIC ACID

ChemComp-DAO:
LAURIC ACID

ChemComp-BUA:
butanoic acid

Source
  • synthetic construct (others)
  • homo sapiens (human)
KeywordsDE NOVO PROTEIN / nanobody / synthetic protein / llama / camelid / TRANSPORT PROTEIN/DE NOVO PROTEIN / TRANSPORT PROTEIN-DE NOVO PROTEIN complex

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