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Structure paper

TitleMultispecific Substrate Recognition in a Proton-Dependent Oligopeptide Transporter.
Journal, issue, pagesStructure, Vol. 26, Page 467-476.e4, Year 2018
Publish dateSep 7, 2017 (structure data deposition date)
AuthorsMartinez Molledo, M. / Quistgaard, E.M. / Flayhan, A. / Pieprzyk, J. / Low, C.
External linksStructure / PubMed:29429879
MethodsX-ray diffraction
Resolution1.95 - 2.66 Å
Structure data

PDB-5oxk:
PepTSt in complex with dipeptide Ala-Gln
Method: X-RAY DIFFRACTION / Resolution: 2.38 Å

PDB-5oxl:
PepTSt in complex with dipeptide Ala-Leu
Method: X-RAY DIFFRACTION / Resolution: 2.66 Å

PDB-5oxm:
PepTSt in complex with dipeptide Asp-Glu
Method: X-RAY DIFFRACTION / Resolution: 2.295 Å

PDB-5oxn:
PepTSt in complex with dipeptide Phe-Ala
Method: X-RAY DIFFRACTION / Resolution: 2.196 Å

PDB-5oxo:
PepTSt apo structure
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-5oxp:
PepTSt in occluded conformation with phosphate ion bound
Method: X-RAY DIFFRACTION / Resolution: 2.372 Å

PDB-5oxq:
PepTSt in complex with HEPES (300 mM)
Method: X-RAY DIFFRACTION / Resolution: 2.195 Å

PDB-6eia:
PepTSt in complex with HEPES (100 mM)
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-78N:
(2R)-2,3-DIHYDROXYPROPYL(7Z)-PENTADEC-7-ENOATE

ChemComp-78M:
(2S)-2,3-DIHYDROXYPROPYL(7Z)-PENTADEC-7-ENOATE

ChemComp-ALA:
ALANINE

ChemComp-GLN:
GLUTAMINE

ChemComp-HOH:
WATER

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

ChemComp-LEU:
LEUCINE

ChemComp-ASP:
ASPARTIC ACID

ChemComp-GLU:
GLUTAMIC ACID

ChemComp-NA:
Unknown entry

ChemComp-PHE:
PHENYLALANINE

ChemComp-CIT:
CITRIC ACID

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

Source
  • streptococcus thermophilus (strain atcc baa-250 / lmg 18311) (bacteria)
  • streptococcus thermophilus (bacteria)
KeywordsTRANSPORT PROTEIN / alpha helical membrane protein / MFS fold / membrane protein / peptide transporter / Alpha-helical membrane protein / Alpha-helical protein / MFS superfamily

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