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-Structure paper
タイトル | A lumenal interrupted helix in human sperm tail microtubules. |
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ジャーナル・号・ページ | Sci Rep, Vol. 8, Issue 1, Page 2727, Year 2018 |
掲載日 | 2018年2月9日 |
![]() | Davide Zabeo / John M Heumann / Cindi L Schwartz / Azusa Suzuki-Shinjo / Garry Morgan / Per O Widlund / Johanna L Höög / ![]() ![]() |
PubMed 要旨 | Eukaryotic flagella are complex cellular extensions involved in many human diseases gathered under the term ciliopathies. Currently, detailed insights on flagellar structure come mostly from studies ...Eukaryotic flagella are complex cellular extensions involved in many human diseases gathered under the term ciliopathies. Currently, detailed insights on flagellar structure come mostly from studies on protists. Here, cryo-electron tomography (cryo-ET) was performed on intact human spermatozoon tails and showed a variable number of microtubules in the singlet region (inside the end-piece). Inside the microtubule plus end, a novel left-handed interrupted helix which extends several micrometers was discovered. This structure was named Tail Axoneme Intra-Lumenal Spiral (TAILS) and binds directly to 11 protofilaments on the internal microtubule wall, in a coaxial fashion with the surrounding microtubule lattice. It leaves a gap over the microtubule seam, which was directly visualized in both singlet and doublet microtubules. We speculate that TAILS may stabilize microtubules, enable rapid swimming or play a role in controlling the swimming direction of spermatozoa. |
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手法 | EM (トモグラフィー) / EM (サブトモグラム平均) |
解像度 | 40.0 Å |
構造データ | ![]() EMDB-14734: Tomogram of human sperm singlet microtubules ![]() EMDB-15450: TAILS structure in human sperm tip singlet microtubules |
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