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Structure paper

TitleStructural Rationale for the Enhanced Catalysis of Nonenzymatic RNA Primer Extension by a Downstream Oligonucleotide.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 140, Page 2829-2840, Year 2018
Publish dateFeb 21, 2017 (structure data deposition date)
AuthorsZhang, W. / Tam, C.P. / Zhou, L. / Oh, S.S. / Wang, J. / Szostak, J.W.
External linksJ. Am. Chem. Soc. / PubMed:29411978
MethodsX-ray diffraction
Resolution1.5 - 3 Å
Structure data

PDB-5ux3:
RNA hairpin structure containing 2-MeImpG monomer analogue and 2-MeImp-oligomer analogue
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-5uz6:
RNA hairpin structure containing 2-MeImp-oligo analogue
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-5v0h:
RNA duplex with 2-MeImpG analogue bound-one binding site
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-5v0j:
RNA duplex with 2-MeImpG analogue bound-2 binding sites
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-5v0o:
RNA hairpin structure containing 2-MeImpG analogue bound
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-5v9z:
RNA hairpin structure containing 2-MeImpG analogue bound
Method: X-RAY DIFFRACTION / Resolution: 2.51 Å

PDB-5vcf:
RNA hairpin structure containing tetraloop/receptor motif, complexed with 2-MeImpG analogue
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-5vci:
RNA hairpin structure containing tetraloop/receptor motif, complexed with 2-MeImpG analogue
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-5vgw:
RNA hairpin structure containing tetraloop/receptor motif
Method: X-RAY DIFFRACTION / Resolution: 2.42 Å

PDB-6az4:
RNA hairpin complex with guanosine dinucleotide ligand G(5')ppp(5')G
Method: X-RAY DIFFRACTION / Resolution: 2.98 Å

PDB-6bmd:
RNA hairpin containing GpppG-oligo to mimic intermediate formed by activated monomer and activated downstream helper
Method: X-RAY DIFFRACTION / Resolution: 3.0 Å

Chemicals

ChemComp-8OS:
5'-O-[(S)-hydroxy(4-methyl-1H-imidazol-5-yl)phosphoryl]guanosine

ChemComp-HOH:
WATER / Water

ChemComp-MG:
Unknown entry

ChemComp-GP3:
DIGUANOSINE-5'-TRIPHOSPHATE

Source
  • synthetic construct (others)
KeywordsRNA / 2-MeIm / analogue / RNA hairpin / molecular evolution / tetraloop/receptor / hairpin / ligation / GpppG / helper / intermediate

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