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-Structure paper
| タイトル | Structure of the 30 kDa HIV-1 RNA Dimerization Signal by a Hybrid Cryo-EM, NMR, and Molecular Dynamics Approach. |
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| ジャーナル・号・ページ | Structure, Vol. 26, Issue 3, Page 490-498.e3, Year 2018 |
| 掲載日 | 2018年3月6日 |
著者 | Kaiming Zhang / Sarah C Keane / Zhaoming Su / Rossitza N Irobalieva / Muyuan Chen / Verna Van / Carly A Sciandra / Jan Marchant / Xiao Heng / Michael F Schmid / David A Case / Steven J Ludtke / Michael F Summers / Wah Chiu / ![]() |
| PubMed 要旨 | Cryoelectron microscopy (cryo-EM) and nuclear magnetic resonance (NMR) spectroscopy are routinely used to determine structures of macromolecules with molecular weights over 65 and under 25 kDa, ...Cryoelectron microscopy (cryo-EM) and nuclear magnetic resonance (NMR) spectroscopy are routinely used to determine structures of macromolecules with molecular weights over 65 and under 25 kDa, respectively. We combined these techniques to study a 30 kDa HIV-1 dimer initiation site RNA ([DIS]; 47 nt/strand). A 9 Å cryo-EM map clearly shows major groove features of the double helix and a right-handed superhelical twist. Simulated cryo-EM maps generated from time-averaged molecular dynamics trajectories (10 ns) exhibited levels of detail similar to those in the experimental maps, suggesting internal structural flexibility limits the cryo-EM resolution. Simultaneous inclusion of the cryo-EM map and H-edited NMR-derived distance restraints during structure refinement generates a structure consistent with both datasets and supporting a flipped-out base within a conserved purine-rich bulge. Our findings demonstrate the power of combining global and local structural information from these techniques for structure determination of modest-sized RNAs. |
リンク | Structure / PubMed:29398526 / PubMed Central |
| 手法 | EM (単粒子) / EM (サブトモグラム平均) / NMR (溶液) |
| 解像度 | 9.0 - 9.3 Å |
| 構造データ | ![]() EMDB-7079: |
| 由来 |
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キーワード | RNA / RNA INERNAL LOOPS / SHEARED GA PAIRS / GU WOBBLE / S-TURN THERMODYNAMICS |
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