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-Structure paper
Title | Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex. |
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Journal, issue, pages | Nat Struct Mol Biol, Vol. 25, Issue 2, Page 135-138, Year 2018 |
Publish date | Jan 22, 2018 |
Authors | Marcello Clerici / Marco Faini / Lena M Muckenfuss / Ruedi Aebersold / Martin Jinek / |
PubMed Abstract | Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and ...Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and polyadenylation specificity factor (CPSF) complex. Here we present a 3.1-Å-resolution cryo-EM structure of a core CPSF module bound to the PAS hexamer motif. The structure reveals the molecular interactions responsible for base-specific recognition, providing a rationale for mechanistic differences between mammalian and yeast 3' polyadenylation. |
External links | Nat Struct Mol Biol / PubMed:29358758 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.07 Å |
Structure data | EMDB-4225: Cryo-EM structure of the human CPSF160-WDR33-CPSF30 complex bound to the PAS AAUAAA motif at 3.1 Angstrom resolution. |
Chemicals | ChemComp-ZN: |
Source |
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Keywords | RNA BINDING PROTEIN / 3' pre-mRNA processing / polyadenylation / CPSF / beta propeller / AAUAAA |