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Structure paper

TitleMolecular mechanism of promoter opening by RNA polymerase III.
Journal, issue, pagesNature, Vol. 553, Issue 7688, Page 295-300, Year 2018
Publish dateJan 17, 2018
AuthorsMatthias K Vorländer / Heena Khatter / Rene Wetzel / Wim J H Hagen / Christoph W Müller /
PubMed AbstractRNA polymerase III (Pol III) and transcription factor IIIB (TFIIIB) assemble together on different promoter types to initiate the transcription of small, structured RNAs. Here we present structures ...RNA polymerase III (Pol III) and transcription factor IIIB (TFIIIB) assemble together on different promoter types to initiate the transcription of small, structured RNAs. Here we present structures of Pol III preinitiation complexes, comprising the 17-subunit Pol III and the heterotrimeric transcription factor TFIIIB, bound to a natural promoter in different functional states. Electron cryo-microscopy reconstructions, varying from 3.7 Å to 5.5 Å resolution, include two early intermediates in which the DNA duplex is closed, an open DNA complex, and an initially transcribing complex with RNA in the active site. Our structures reveal an extremely tight, multivalent interaction between TFIIIB and promoter DNA, and explain how TFIIIB recruits Pol III. Together, TFIIIB and Pol III subunit C37 activate the intrinsic transcription factor-like activity of the Pol III-specific heterotrimer to initiate the melting of double-stranded DNA, in a mechanism similar to that of the Pol II system.
External linksNature / PubMed:29345638 / PubMed Central
MethodsEM (single particle)
Resolution3.7 - 5.5 Å
Structure data

EMDB-4180, PDB-6f40:
RNA Polymerase III open complex
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-4181, PDB-6f41:
RNA Polymerase III initially transcribing complex
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-4182, PDB-6f42:
RNA Polymerase III closed complex CC1.
Method: EM (single particle) / Resolution: 5.5 Å

EMDB-4183, PDB-6f44:
RNA Polymerase III closed complex CC2.
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-4184:
RNA Polymerase III pre-initiation complex
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • Baker's yeast (brewer's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • saccharomyces cerevisiae s288c (yeast)
KeywordsTRANSCRIPTION / RNA Polymerase III / TFIIIB / Pre-initiation complex / Brf1 / Bdp1 / TBP / Pol III / Enzyme / Closed complex

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