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TitleStructural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 114, Issue 52, Page 13708-13713, Year 2017
Publish dateDec 26, 2017
AuthorsYingyuan Sun / Aaron P Roznowski / Joshua M Tokuda / Thomas Klose / Alexander Mauney / Lois Pollack / Bentley A Fane / Michael G Rossmann /
PubMed AbstractUnlike tailed bacteriophages, which use a preformed tail for transporting their genomes into a host bacterium, the ssDNA bacteriophage ΦX174 is tailless. Using cryo-electron microscopy and time- ...Unlike tailed bacteriophages, which use a preformed tail for transporting their genomes into a host bacterium, the ssDNA bacteriophage ΦX174 is tailless. Using cryo-electron microscopy and time-resolved small-angle X-ray scattering, we show that lipopolysaccharides (LPS) form bilayers that interact with ΦX174 at an icosahedral fivefold vertex and induce single-stranded (ss) DNA genome ejection. The structures of ΦX174 complexed with LPS have been determined for the pre- and post-ssDNA ejection states. The ejection is initiated by the loss of the G protein spike that encounters the LPS, followed by conformational changes of two polypeptide loops on the major capsid F proteins. One of these loops mediates viral attachment, and the other participates in making the fivefold channel at the vertex contacting the LPS.
External linksProc Natl Acad Sci U S A / PubMed:29229840 / PubMed Central
MethodsEM (single particle)
Resolution9.9 Å
Structure data

EMDB-7033:
Emptied phiX174 complexed with lipopolysaccharides after DNA ejection
Method: EM (single particle) / Resolution: 9.9 Å

EMDB-8862:
Full phiX174 complexed with lipopolysaccharides before DNA ejection
Method: EM (single particle) / Resolution: 9.9 Å

Source
  • Enterobacteria phage phiX174 isolate Sanger (virus)
  • Salmonella enterica (bacteria)

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