EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Proteasomes tether to two distinct sites at the nuclear pore complex.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 114, Issue 52, Page 13726-13731, Year 2017
掲載日	2017年12月26日
著者	Sahradha Albert / Miroslava Schaffer / Florian Beck / Shyamal Mosalaganti / Shoh Asano / Henry F Thomas / Jürgen M Plitzko / Martin Beck / Wolfgang Baumeister / Benjamin D Engel /
PubMed 要旨	The partitioning of cellular components between the nucleus and cytoplasm is the defining feature of eukaryotic life. The nuclear pore complex (NPC) selectively gates the transport of macromolecules ...The partitioning of cellular components between the nucleus and cytoplasm is the defining feature of eukaryotic life. The nuclear pore complex (NPC) selectively gates the transport of macromolecules between these compartments, but it is unknown whether surveillance mechanisms exist to reinforce this function. By leveraging in situ cryo-electron tomography to image the native cellular environment of , we observed that nuclear 26S proteasomes crowd around NPCs. Through a combination of subtomogram averaging and nanometer-precision localization, we identified two classes of proteasomes tethered via their Rpn9 subunits to two specific NPC locations: binding sites on the NPC basket that reflect its eightfold symmetry and more abundant binding sites at the inner nuclear membrane that encircle the NPC. These basket-tethered and membrane-tethered proteasomes, which have similar substrate-processing state frequencies as proteasomes elsewhere in the cell, are ideally positioned to regulate transcription and perform quality control of both soluble and membrane proteins transiting the NPC.
リンク	Proc Natl Acad Sci U S A / PubMed:29229809 / PubMed Central
手法	EM (サブトモグラム平均) / EM (トモグラフィー)
解像度	23.81 - 36.48 Å
構造データ	EMDB-3936: In situ subtomogram average of the Chlamydomonas membrane-tethered 26S proteasome 手法: EM (サブトモグラム平均) / 解像度: 24.87 Å EMDB-3937: In situ subtomogram average of the Chlamydomonas basket-tethered 26S proteasome 手法: EM (サブトモグラム平均) / 解像度: 34.48 Å EMDB-3938: In situ subtomogram average of the Chlamydomonas free 26S proteasome 手法: EM (サブトモグラム平均) / 解像度: 23.81 Å EMDB-3939: Local refinement of the extra density that tethers 26S proteasomes to the NPC basket 手法: EM (サブトモグラム平均) / 解像度: 36.48 Å EMDB-3940: Local refinement of the extra density that tethers 26S proteasomes to the inner nuclear membrane 手法: EM (サブトモグラム平均) / 解像度: 27.36 Å EMDB-3967: In situ cryo-electron tomogram from Chlamydomonas reinhardtii of the cellular environment around the nuclear envelope 手法: EM (トモグラフィー)
由来	Chlamydomonas reinhardtii (クラミドモナス)