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TitleProteasomes tether to two distinct sites at the nuclear pore complex.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 114, Issue 52, Page 13726-13731, Year 2017
Publish dateDec 26, 2017
AuthorsSahradha Albert / Miroslava Schaffer / Florian Beck / Shyamal Mosalaganti / Shoh Asano / Henry F Thomas / Jürgen M Plitzko / Martin Beck / Wolfgang Baumeister / Benjamin D Engel /
PubMed AbstractThe partitioning of cellular components between the nucleus and cytoplasm is the defining feature of eukaryotic life. The nuclear pore complex (NPC) selectively gates the transport of macromolecules ...The partitioning of cellular components between the nucleus and cytoplasm is the defining feature of eukaryotic life. The nuclear pore complex (NPC) selectively gates the transport of macromolecules between these compartments, but it is unknown whether surveillance mechanisms exist to reinforce this function. By leveraging in situ cryo-electron tomography to image the native cellular environment of , we observed that nuclear 26S proteasomes crowd around NPCs. Through a combination of subtomogram averaging and nanometer-precision localization, we identified two classes of proteasomes tethered via their Rpn9 subunits to two specific NPC locations: binding sites on the NPC basket that reflect its eightfold symmetry and more abundant binding sites at the inner nuclear membrane that encircle the NPC. These basket-tethered and membrane-tethered proteasomes, which have similar substrate-processing state frequencies as proteasomes elsewhere in the cell, are ideally positioned to regulate transcription and perform quality control of both soluble and membrane proteins transiting the NPC.
External linksProc Natl Acad Sci U S A / PubMed:29229809 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution23.81 - 36.48 Å
Structure data

EMDB-3936:
In situ subtomogram average of the Chlamydomonas membrane-tethered 26S proteasome
Method: EM (subtomogram averaging) / Resolution: 24.87 Å

EMDB-3937:
In situ subtomogram average of the Chlamydomonas basket-tethered 26S proteasome
Method: EM (subtomogram averaging) / Resolution: 34.48 Å

EMDB-3938:
In situ subtomogram average of the Chlamydomonas free 26S proteasome
Method: EM (subtomogram averaging) / Resolution: 23.81 Å

EMDB-3939:
Local refinement of the extra density that tethers 26S proteasomes to the NPC basket
Method: EM (subtomogram averaging) / Resolution: 36.48 Å

EMDB-3940:
Local refinement of the extra density that tethers 26S proteasomes to the inner nuclear membrane
Method: EM (subtomogram averaging) / Resolution: 27.36 Å

EMDB-3967:
In situ cryo-electron tomogram from Chlamydomonas reinhardtii of the cellular environment around the nuclear envelope
Method: EM (tomography)

Source
  • Chlamydomonas reinhardtii (plant)

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