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Structure paper

TitleDesign and applications of a clamp for Green Fluorescent Protein with picomolar affinity.
Journal, issue, pagesSci Rep, Vol. 7, Page 16292-16292, Year 2017
Publish dateNov 3, 2016 (structure data deposition date)
AuthorsHansen, S. / Stuber, J.C. / Ernst, P. / Koch, A. / Bojar, D. / Batyuk, A. / Pluckthun, A.
External linksSci Rep / PubMed:29176615
MethodsX-ray diffraction
Resolution1.4 - 2.5 Å
Structure data

PDB-5ma3:
GFP-binding DARPin fusion gc_R11
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-5ma4:
GFP-binding DARPin fusion gc_K7
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-5ma5:
GFP-binding DARPin fusion gc_K11
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-5ma6:
GFP-binding DARPin 3G124nc
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-5ma8:
GFP-binding DARPin 3G124nc
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

PDB-5ma9:
GFP-binding DARPin fusion gc_R11
Method: X-RAY DIFFRACTION / Resolution: 1.57 Å

PDB-5mad:
GFP-binding DARPin 3G61
Method: X-RAY DIFFRACTION / Resolution: 1.53 Å

PDB-5mak:
GFP-binding DARPin fusion gc_R7
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-CIT:
CITRIC ACID

ChemComp-IPA:
ISOPROPYL ALCOHOL / alkaloid*YM

ChemComp-NA:
Unknown entry

ChemComp-PO4:
PHOSPHATE ION

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • aequorea victoria (jellyfish)
  • synthetic construct (others)
KeywordsFLUORESCENT PROTEIN / green fluorescent protein / designed ankyrin protein / designed ankyrin repeat protein

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