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TitleThe structure of the COPI coat determined within the cell.
Journal, issue, pagesElife, Vol. 6, Year 2017
Publish dateNov 17, 2017
AuthorsYury S Bykov / Miroslava Schaffer / Svetlana O Dodonova / Sahradha Albert / Jürgen M Plitzko / Wolfgang Baumeister / Benjamin D Engel / John Ag Briggs /
PubMed AbstractCOPI-coated vesicles mediate trafficking within the Golgi apparatus and from the Golgi to the endoplasmic reticulum. The structures of membrane protein coats, including COPI, have been extensively ...COPI-coated vesicles mediate trafficking within the Golgi apparatus and from the Golgi to the endoplasmic reticulum. The structures of membrane protein coats, including COPI, have been extensively studied with reconstitution systems using purified components. Previously we have determined a complete structural model of the reconstituted COPI coat (Dodonova et al., 2017). Here, we applied cryo-focused ion beam milling, cryo-electron tomography and subtomogram averaging to determine the native structure of the COPI coat within vitrified cells. The native algal structure resembles the mammalian structure, but additionally reveals cargo bound beneath β'-COP. We find that all coat components disassemble simultaneously and relatively rapidly after budding. Structural analysis , maintaining Golgi topology, shows that vesicles change their size, membrane thickness, and cargo content as they progress from to , but the structure of the coat machinery remains constant.
External linksElife / PubMed:29148969 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution20.0 - 33.0 Å
Structure data

EMDB-3968:
The in situ structure of the Chlamydomonas COPI coat: average of all data
Method: EM (subtomogram averaging) / Resolution: 20.0 Å

EMDB-3969:
The in situ structure of the Chlamydomonas COPI coat: average from buds
Method: EM (subtomogram averaging) / Resolution: 23.0 Å

EMDB-3970:
The in situ structure of the Chlamydomonas COPI coat: average from vesicles
Method: EM (subtomogram averaging) / Resolution: 23.0 Å

EMDB-3971:
The in situ structure of the Chlamydomonas COPI coat: average from the cis-Golgi region
Method: EM (subtomogram averaging) / Resolution: 24.0 Å

EMDB-3972:
The in situ structure of the Chlamydomonas COPI coat: average from the medial/trans-Golgi region
Method: EM (subtomogram averaging) / Resolution: 23.0 Å

EMDB-3973:
The in situ structure of the Chlamydomonas COPI coat: average from the trans-Golgi/TGN region
Method: EM (subtomogram averaging) / Resolution: 24.0 Å

EMDB-3974:
The in situ structure of the Chlamydomonas COPI coat linkage I
Method: EM (subtomogram averaging) / Resolution: 33.0 Å

EMDB-3975:
The in situ structure of the Chlamydomonas COPI coat linkage II
Method: EM (subtomogram averaging) / Resolution: 32.0 Å

EMDB-3976:
The in situ structure of the Chlamydomonas COPI coat linkage IV
Method: EM (subtomogram averaging) / Resolution: 32.0 Å

EMDB-3977:
Cryo-electron tomogram of the Chlamydomonas reinhardtii Golgi apparatus
Method: EM (tomography)

Source
  • Chlamydomonas reinhardtii (plant)

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