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Title | Cryo-EM reconstruction of the chlororibosome to 3.2 Å resolution within 24 h. |
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Journal, issue, pages | IUCrJ, Vol. 4, Issue Pt 6, Page 723-727, Year 2017 |
Publish date | Nov 1, 2017 |
Authors | Björn O Forsberg / Shintaro Aibara / Dari Kimanius / Bijoya Paul / Erik Lindahl / Alexey Amunts / |
PubMed Abstract | The introduction of direct detectors and the automation of data collection in cryo-EM have led to a surge in data, creating new opportunities for advancing computational processing. In particular, on- ...The introduction of direct detectors and the automation of data collection in cryo-EM have led to a surge in data, creating new opportunities for advancing computational processing. In particular, on-the-fly workflows that connect data collection with three-dimensional reconstruction would be valuable for more efficient use of cryo-EM and its application as a sample-screening tool. Here, accelerated on-the-fly analysis is reported with optimized organization of the data-processing tools, image acquisition and particle alignment that make it possible to reconstruct the three-dimensional density of the 70S chlororibosome to 3.2 Å resolution within 24 h of tissue harvesting. It is also shown that it is possible to achieve even faster processing at comparable quality by imposing some limits to data use, as illustrated by a 3.7 Å resolution map that was obtained in only 80 min on a desktop computer. These on-the-fly methods can be employed as an assessment of data quality from small samples and extended to high-throughput approaches. |
External links | IUCrJ / PubMed:29123673 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.2 - 4.2 Å |
Structure data | EMDB-3806: EMDB-3807: EMDB-3808: |
Source |
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