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TitleStructural insights into DNA cleavage activation of CRISPR-Cas9 system.
Journal, issue, pagesNat Commun, Vol. 8, Issue 1, Page 1375, Year 2017
Publish dateNov 9, 2017
AuthorsCong Huai / Gan Li / Ruijie Yao / Yingyi Zhang / Mi Cao / Liangliang Kong / Chenqiang Jia / Hui Yuan / Hongyan Chen / Daru Lu / Qiang Huang /
PubMed AbstractCRISPR-Cas9 technology has been widely used for genome engineering. Its RNA-guided endonuclease Cas9 binds specifically to target DNA and then cleaves the two DNA strands with HNH and RuvC nuclease ...CRISPR-Cas9 technology has been widely used for genome engineering. Its RNA-guided endonuclease Cas9 binds specifically to target DNA and then cleaves the two DNA strands with HNH and RuvC nuclease domains. However, structural information regarding the DNA cleavage-activating state of two nuclease domains remains sparse. Here, we report a 5.2 Å cryo-EM structure of Cas9 in complex with sgRNA and target DNA. This structure reveals a conformational state of Cas9 in which the HNH domain is closest to the DNA cleavage site. Compared with two known HNH states, our structure shows that the HNH active site moves toward the cleavage site by about 25 and 13 Å, respectively. In combination with EM-based molecular dynamics simulations, we show that residues of the nuclease domains in our structure could form cleavage-compatible conformations with the target DNA. Together, these results strongly suggest that our cryo-EM structure resembles a DNA cleavage-activating architecture of Cas9.
External linksNat Commun / PubMed:29123204 / PubMed Central
MethodsEM (single particle)
Resolution5.2 Å
Structure data

8236

5y36

EMDB-8236, PDB-5y36:
Cryo-EM structure of SpCas9-sgRNA-DNA ternary complex
Method: EM (single particle) / Resolution: 5.2 Å

Chemicals

ChemComp-MG:
Unknown entry

Source
  • Streptococcus pyogenes (bacteria)
  • streptococcus pyogenes serotype m1 (bacteria)
  • mus musculus (house mouse)
KeywordsHYDROLASE/RNA/DNA / Genome editting / CRIPSR-Cas9 / DNA cleavage mechanism / HYDROLASE-DNA-RNA complex / HYDROLASE-RNA-DNA complex

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