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Structure paper

TitleStructural Basis of Mec1-Ddc2-RPA Assembly and Activation on Single-Stranded DNA at Sites of Damage.
Journal, issue, pagesMol. Cell, Vol. 68, Page 431-445.e5, Year 2017
Publish dateJul 28, 2017 (structure data deposition date)
AuthorsDeshpande, I. / Seeber, A. / Shimada, K. / Keusch, J.J. / Gut, H. / Gasser, S.M.
External linksMol. Cell / PubMed:29033322
MethodsX-ray diffraction
Resolution1.94 - 2.38 Å
Structure data

PDB-5omb:
Crystal structure of K. lactis Ddc2 N-terminus in complex with S. cerevisiae Rfa1 N-OB domain
Method: X-RAY DIFFRACTION / Resolution: 1.94 Å

PDB-5omc:
Crystal structure of K. lactis Ddc2 N-terminus in complex with S. cerevisiae Rfa1 (K45E mutant) N-OB domain
Method: X-RAY DIFFRACTION / Resolution: 2.38 Å

PDB-5omd:
Crystal structure of S. cerevisiae Ddc2 N-terminal coiled-coil domain
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • kluyveromyces lactis (strain atcc 8585 / cbs 2359 / dsm 70799 / nbrc 1267 / nrrl y-1140 / wm37) (yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
  • kluyveromyces lactis (yeast)
KeywordsPROTEIN BINDING / Oligonucleotide-binding fold / coiled-coil domain / complex / nucleus / mutant / coiled-coil / dimerization

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