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TitleStructural insights into species-specific features of the ribosome from the human pathogen Mycobacterium tuberculosis.
Journal, issue, pagesNucleic Acids Res, Vol. 45, Issue 18, Page 10884-10894, Year 2017
Publish dateOct 13, 2017
AuthorsKailu Yang / Jeng-Yih Chang / Zhicheng Cui / Xiaojun Li / Ran Meng / Lijun Duan / Jirapat Thongchol / Joanita Jakana / Christoph M Huwe / James C Sacchettini / Junjie Zhang /
PubMed AbstractRibosomes from Mycobacterium tuberculosis (Mtb) possess species-specific ribosomal RNA (rRNA) expansion segments and ribosomal proteins (rProtein). Here, we present the near-atomic structures of the ...Ribosomes from Mycobacterium tuberculosis (Mtb) possess species-specific ribosomal RNA (rRNA) expansion segments and ribosomal proteins (rProtein). Here, we present the near-atomic structures of the Mtb 50S ribosomal subunit and the complete Mtb 70S ribosome, solved by cryo-electron microscopy. Upon joining of the large and small ribosomal subunits, a 100-nt long expansion segment of the Mtb 23S rRNA, named H54a or the 'handle', switches interactions from with rRNA helix H68 and rProtein uL2 to with rProtein bS6, forming a new intersubunit bridge 'B9'. In Mtb 70S, bridge B9 is mostly maintained, leading to correlated motions among the handle, the L1 stalk and the small subunit in the rotated and non-rotated states. Two new protein densities were discovered near the decoding center and the peptidyl transferase center, respectively. These results provide a structural basis for studying translation in Mtb as well as developing new tuberculosis drugs.
External linksNucleic Acids Res / PubMed:28977617 / PubMed Central
MethodsEM (single particle)
Resolution3.7 - 7.1 Å
Structure data

8641

5v7q

EMDB-8641: Cryo-EM structure of the large ribosomal subunit from Mycobacterium tuberculosis
PDB-5v7q: Cryo-EM structure of the large ribosomal subunit from Mycobacterium tuberculosis bound with a potent linezolid analog
Method: EM (single particle) / Resolution: 3.7 Å

8645

5v93

EMDB-8645, PDB-5v93:
Cryo-EM structure of the 70S ribosome from Mycobacterium tuberculosis bound with Capreomycin
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-8646:
Locally-refined cryo-EM structure of the small ribosomal subunit from Mycobacterium tuberculosis
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-8647:
Cryo-EM structure of the 70S ribosome from Mycobacterium smegmatis
Method: EM (single particle) / Resolution: 7.1 Å

EMDB-8648:
Focused classification of the 70S ribosome from Mycobacterium tuberculosis
Method: EM (single particle) / Resolution: 6.6 Å

EMDB-8649:
Cryo-EM structure of the large ribosomal subunit from Mycobacterium tuberculosis at 1 mM magnesium concentration
Method: EM (single particle) / Resolution: 6.0 Å

Chemicals

ChemComp-917:
N-({(5S)-2-oxo-3-[4-(1,3-thiazol-5-yl)phenyl]-1,3-oxazolidin-5-yl}methyl)acetamide

Source
  • mycobacterium tuberculosis (bacteria)
  • streptomyces puniceus (bacteria)
  • Mycobacterium smegmatis (bacteria)
KeywordsRIBOSOME / RNA dynamics / RNA complex

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