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TitleThe Conformational Flexibility of the Acyltransferase from the Disorazole Polyketide Synthase Is Revealed by an X-ray Free-Electron Laser Using a Room-Temperature Sample Delivery Method for Serial Crystallography.
Journal, issue, pagesBiochemistry, Vol. 56, Page 4751-4756, Year 2017
Publish dateAug 17, 2017 (structure data deposition date)
AuthorsMathews, I.I. / Allison, K. / Robbins, T. / Lyubimov, A.Y. / Uervirojnangkoorn, M. / Brunger, A.T. / Khosla, C. / DeMirci, H. / McPhillips, S.E. / Hollenbeck, M. ...Mathews, I.I. / Allison, K. / Robbins, T. / Lyubimov, A.Y. / Uervirojnangkoorn, M. / Brunger, A.T. / Khosla, C. / DeMirci, H. / McPhillips, S.E. / Hollenbeck, M. / Soltis, M. / Cohen, A.E.
External linksBiochemistry / PubMed:28832129
MethodsX-ray diffraction
Resolution1.63 - 2.5 Å
Structure data

PDB-6apf:
Trans-acting transferase from Disorazole synthase complexed with Citrate.
Method: X-RAY DIFFRACTION / Resolution: 1.63 Å

PDB-6apg:
Trans-acting transferase from Disorazole synthase with malonate
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

PDB-6apk:
Trans-acting transferase from Disorazole synthase solved by serial femtosecond XFEL crystallography
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-6apm:
Hen egg-white lysozyme (WT), solved with serial millisecond crystallography using synchrotron radiation
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

Chemicals

ChemComp-CIT:
CITRIC ACID

ChemComp-GOL:
GLYCEROL

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-MLI:
MALONATE ION

ChemComp-CA:
Unknown entry

ChemComp-NA:
Unknown entry

Source
  • sorangium cellulosum (bacteria)
  • gallus gallus (chicken)
KeywordsTRANSFERASE / acyl transferase / Disorazole synthase / citrate complex / polyketide synthase / malonate complex / XFEL crystal structure / serial femtosecond crystallography / HYDROLASE / HEWL / lysozyme / serial millisecond crystallography

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