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TitleKatanin spiral and ring structures shed light on power stroke for microtubule severing.
Journal, issue, pagesNat Struct Mol Biol, Vol. 24, Issue 9, Page 717-725, Year 2017
Publish dateAug 7, 2017
AuthorsElena Zehr / Agnieszka Szyk / Grzegorz Piszczek / Ewa Szczesna / Xiaobing Zuo / Antonina Roll-Mecak /
PubMed AbstractMicrotubule-severing enzymes katanin, spastin and fidgetin are AAA ATPases important for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. Because of a lack ...Microtubule-severing enzymes katanin, spastin and fidgetin are AAA ATPases important for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. Because of a lack of known 3D structures for these enzymes, their mechanism of action has remained poorly understood. Here we report the X-ray crystal structure of the monomeric AAA katanin module from Caenorhabditis elegans and cryo-EM reconstructions of the hexamer in two conformations. The structures reveal an unexpected asymmetric arrangement of the AAA domains mediated by structural elements unique to microtubule-severing enzymes and critical for their function. The reconstructions show that katanin cycles between open spiral and closed ring conformations, depending on the ATP occupancy of a gating protomer that tenses or relaxes interprotomer interfaces. Cycling of the hexamer between these conformations would provide the power stroke for microtubule severing.
External linksNat Struct Mol Biol / PubMed:28783150 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution3.3 - 6.0 Å
Structure data

8794

5wc0

EMDB-8794, PDB-5wc0:
katanin hexamer in spiral conformation
Method: EM (single particle) / Resolution: 4.4 Å

8796

5wcb

EMDB-8796, PDB-5wcb:
Katanin hexamer in the ring conformation
Method: EM (single particle) / Resolution: 6.0 Å

PDB-5wc1:
katanin AAA ATPase domain
Method: X-RAY DIFFRACTION / Resolution: 3.3 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-SO4:
SULFATE ION / Sulfate

Source
  • caenorhabditis elegans (invertebrata)
KeywordsMOTOR PROTEIN / Microtubule cytoskeleton / microtubule-severing enzyme / AAA ATPase / p60 / CYTOSOLIC PROTEIN / microtubule severing enzyme / microtubule severing protein

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