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TitleStructure-based protein engineering for thermostable and alkaliphilic enhancement of endo-beta-1,4-xylanase for applications in pulp bleaching
Journal, issue, pagesJ. Biotechnol., Vol. 259, Page 95-102, Year 2017
Publish dateSep 1, 2016 (structure data deposition date)
AuthorsBoonyapakron, K. / Jaruwat, A. / Liwnaree, B. / Nimchua, T. / Champreda, V. / Chitnumsub, P.
External linksJ. Biotechnol. / PubMed:28774672
MethodsX-ray diffraction
Resolution1.4 - 1.97 Å
Structure data

PDB-5gv1:
Crystal structure of ENZbleach xylanase wild type
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-5gy8:
Crystal structure of ENZbleach xylanase T28C+T60C mutant
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-5gy9:
Crystal structure of ENZbleach xylanase A74C+G84C mutant
Method: X-RAY DIFFRACTION / Resolution: 1.94 Å

PDB-5gya:
Crystal structure of ENZbleach xylanase V5N+V6N+K7R+K223R+K227R mutant
Method: X-RAY DIFFRACTION / Resolution: 1.93 Å

PDB-5gyb:
Crystal structure of ENZbleach xylanase V5N+V6N+K7R+K223R+K227R and T28C+T60C mutant
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-5gyc:
Crystal structure of ENZbleach xylanase K73R+K185R and T28C+T60C mutant
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-5gye:
Crystal structure of ENZbleach xylanase T28C+T60C and T77C+E249C mutant
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-5gyf:
Crystal structure of ENZbleach xylanase T28C+T60C+L59F mutant
Method: X-RAY DIFFRACTION / Resolution: 1.94 Å

PDB-5gyg:
Crystal structure of ENZbleach xylanase T28C+T60C mutant with three N-teminal residue truncation
Method: X-RAY DIFFRACTION / Resolution: 1.74 Å

PDB-5gyh:
Crystal structure of ENZbleach xylanase T28C+T60C+T48F+L59F mutant
Method: X-RAY DIFFRACTION / Resolution: 1.86 Å

PDB-5gyi:
Crystal structure of ENZbleach xylanase V176C+E220C mutant
Method: X-RAY DIFFRACTION / Resolution: 1.97 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • termite gut metagenome (others)
KeywordsHYDROLASE / Endo-1 / 4-beta-xylanase / GH11 xylanase

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