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-Structure paper
タイトル | Morphology of the archaellar motor and associated cytoplasmic cone in . |
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ジャーナル・号・ページ | EMBO Rep, Vol. 18, Issue 9, Page 1660-1670, Year 2017 |
掲載日 | 2017年7月19日 |
著者 | Ariane Briegel / Catherine M Oikonomou / Yi-Wei Chang / Andreas Kjær / Audrey N Huang / Ki Woo Kim / Debnath Ghosal / Hong H Nguyen / Dorothy Kenny / Rachel R Ogorzalek Loo / Robert P Gunsalus / Grant J Jensen / |
PubMed 要旨 | Archaeal swimming motility is driven by archaella: rotary motors attached to long extracellular filaments. The structure of these motors, and particularly how they are anchored in the absence of a ...Archaeal swimming motility is driven by archaella: rotary motors attached to long extracellular filaments. The structure of these motors, and particularly how they are anchored in the absence of a peptidoglycan cell wall, is unknown. Here, we use electron cryotomography to visualize the archaellar basal body in KOD1. Compared to the homologous bacterial type IV pilus (T4P), we observe structural similarities as well as several unique features. While the position of the cytoplasmic ATPase appears conserved, it is not braced by linkages that extend upward through the cell envelope as in the T4P, but rather by cytoplasmic components that attach it to a large conical frustum up to 500 nm in diameter at its base. In addition to anchoring the lophotrichous bundle of archaella, the conical frustum associates with chemosensory arrays and ribosome-excluding material and may function as a polar organizing center for the coccoid cells. |
リンク | EMBO Rep / PubMed:28729461 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 40.0 Å |
構造データ | EMDB-8603: |
由来 |
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