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TitleThe Complete Structure of the Mycobacterium smegmatis 70S Ribosome.
Journal, issue, pagesCell Rep, Vol. 20, Issue 1, Page 149-160, Year 2017
Publish dateJul 5, 2017
AuthorsJendrik Hentschel / Chloe Burnside / Ingrid Mignot / Marc Leibundgut / Daniel Boehringer / Nenad Ban /
PubMed AbstractThe ribosome carries out the synthesis of proteins in every living cell. It consequently represents a frontline target in anti-microbial therapy. Tuberculosis ranks among the leading causes of death ...The ribosome carries out the synthesis of proteins in every living cell. It consequently represents a frontline target in anti-microbial therapy. Tuberculosis ranks among the leading causes of death worldwide, due in large part to the combination of difficult-to-treat latency and antibiotic resistance. Here, we present the 3.3-Å cryo-EM structure of the 70S ribosome of Mycobacterium smegmatis, a close relative to the human pathogen Mycobacterium tuberculosis. The structure reveals two additional ribosomal proteins and localizes them to the vicinity of drug-target sites in both the catalytic center and the decoding site of the ribosome. Furthermore, we visualized actinobacterium-specific rRNA and protein expansions that extensively remodel the ribosomal surface with implications for polysome organization. Our results provide a foundation for understanding the idiosyncrasies of mycobacterial translation and reveal atomic details of the structure that will facilitate the design of anti-tubercular therapeutics.
External linksCell Rep / PubMed:28683309
MethodsEM (single particle)
Resolution3.18 - 3.45 Å
Structure data

3748

5o5j

EMDB-3748: Cryo-EM reconstruction of the small subunit of the Mycobacterium smegmatis ribosome
PDB-5o5j: Structure of the 30S small ribosomal subunit from Mycobacterium smegmatis
Method: EM (single particle) / Resolution: 3.45 Å

3750

5o60

EMDB-3750: Cryo-EM reconstruction of the large subunit of the Mycobacterium smegmatis ribosome
PDB-5o60: Structure of the 50S large ribosomal subunit from Mycobacterium smegmatis
Method: EM (single particle) / Resolution: 3.18 Å

3751

5o61

EMDB-3751: Cryo-EM reconstruction of the Mycobacterium smegmatis 70S ribosome
PDB-5o61: The complete structure of the Mycobacterium smegmatis 70S ribosome
Method: EM (single particle) / Resolution: 3.31 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-PHE:
PHENYLALANINE / Phenylalanine

Source
  • mycobacterium smegmatis str. mc2 155 (bacteria)
KeywordsRIBOSOME / translation

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