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TitleInhibition Mechanism of an Anti-CRISPR Suppressor AcrIIA4 Targeting SpyCas9.
Journal, issue, pagesMol. Cell, Vol. 67, Page 117-127.e5, Year 2017
Publish dateMay 21, 2017 (structure data deposition date)
AuthorsYang, H. / Patel, D.J.
External linksMol. Cell / PubMed:28602637
MethodsX-ray diffraction
Resolution2.598 Å
Structure data

PDB-5vw1:
Crystal structure of SpyCas9-sgRNA-AcrIIA4 ternary complex
Method: X-RAY DIFFRACTION / Resolution: 2.598 Å

Chemicals

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • streptococcus pyogenes serotype m1 (bacteria)
  • listeria monocytogenes serotype 4a (strain m7) (bacteria)
  • listeria monocytogenes (bacteria)
KeywordsHYDROLASE / Type II CRISPR-Cas endonculease: Cas9: Structure: Endonuclease: anti-CRISPR protein: Inhibition of Cas9: RuvC catalytic pocket: Sequence-specific PAM recognition: Genome editing tool

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