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TitleAssembly, maturation and three-dimensional helical structure of the teratogenic rubella virus.
Journal, issue, pagesPLoS Pathog, Vol. 13, Issue 6, Page e1006377, Year 2017
Publish dateJun 2, 2017
AuthorsVidya Mangala Prasad / Thomas Klose / Michael G Rossmann /
PubMed AbstractViral infections during pregnancy are a significant cause of infant morbidity and mortality. Of these, rubella virus infection is a well-substantiated example that leads to miscarriages or severe ...Viral infections during pregnancy are a significant cause of infant morbidity and mortality. Of these, rubella virus infection is a well-substantiated example that leads to miscarriages or severe fetal defects. However, structural information about the rubella virus has been lacking due to the pleomorphic nature of the virions. Here we report a helical structure of rubella virions using cryo-electron tomography. Sub-tomogram averaging of the surface spikes established the relative positions of the viral glycoproteins, which differed from the earlier icosahedral models of the virus. Tomographic analyses of in vitro assembled nucleocapsids and virions provide a template for viral assembly. Comparisons of immature and mature virions show large rearrangements in the glycoproteins that may be essential for forming the infectious virions. These results present the first known example of a helical membrane-enveloped virus, while also providing a structural basis for its assembly and maturation pathway.
External linksPLoS Pathog / PubMed:28575072 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution11.1 - 35.0 Å
Structure data

8248

5khc

EMDB-8248: Subtomogram-averaged surface glycoprotein spike density of rubella virus
PDB-5khc: Structure of rubella virus E1 glycoprotein ectodomain fitted into sub-tomogram averaged surface spike density of rubella virus
Method: EM (subtomogram averaging) / Resolution: 11.1 Å

8249

5khe

EMDB-8249: Subtomogram-averaged map of a single rubella virus capsid unit
PDB-5khe: Fitted structure of rubella virus capsid protein
Method: EM (subtomogram averaging) / Resolution: 35.0 Å

8250

5khf

EMDB-8250: Subtomogram-averaged map of a single rubella virus capsid unit
PDB-5khf: Fitted structure of rubella virus capsid protein
Method: EM (subtomogram averaging) / Resolution: 35.0 Å

EMDB-8251:
Subtomogram-averaged map of in vitro assembled rubella virus capsid protein tetramer complex
Method: EM (subtomogram averaging) / Resolution: 35.0 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • rubella virus
  • Rubella virus strain M33
KeywordsVIRAL PROTEIN / Rubella virus / surface glycoprotein spike / E1-E2 heterodimer / rubella virus capsid protein

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