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TitleAlternate binding modes of anti-CRISPR viral suppressors AcrF1/2 to Csy surveillance complex revealed by cryo-EM structures.
Journal, issue, pagesCell Res, Vol. 27, Issue 7, Page 853-864, Year 2017
Publish dateJun 2, 2017
AuthorsRuchao Peng / Ying Xu / Tengfei Zhu / Ningning Li / Jianxun Qi / Yan Chai / Min Wu / Xinzheng Zhang / Yi Shi / Peiyi Wang / Jiawei Wang / Ning Gao / George Fu Gao /
PubMed AbstractBacteriophages encode anti-CRISPR suppressors to counteract the CRISPR/Cas immunity of their bacterial hosts, thus facilitating their survival and replication. Previous studies have shown that two ...Bacteriophages encode anti-CRISPR suppressors to counteract the CRISPR/Cas immunity of their bacterial hosts, thus facilitating their survival and replication. Previous studies have shown that two phage-encoded anti-CRISPR proteins, AcrF1 and AcrF2, suppress the type I-F CRISPR/Cas system of Pseudomonas aeruginosa by preventing target DNA recognition by the Csy surveillance complex, but the precise underlying mechanism was unknown. Here we present the structure of AcrF1/2 bound to the Csy complex determined by cryo-EM single-particle reconstruction. By structural analysis, we found that AcrF1 inhibits target DNA recognition of the Csy complex by interfering with base pairing between the DNA target strand and crRNA spacer. In addition, multiple copies of AcrF1 bind to the Csy complex with different modes when working individually or cooperating with AcrF2, which might exclude target DNA binding through different mechanisms. Together with previous reports, we provide a comprehensive working scenario for the two anti-CRISPR suppressors, AcrF1 and AcrF2, which silence CRISPR/Cas immunity by targeting the Csy surveillance complex.
External linksCell Res / PubMed:28574055 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 5.3 Å
Structure data

EMDB-6728:
Anti-CRISPR protein AcrF1/2 bound to Csy surveillance complex with a 20nt spacer crRNA
Method: EM (single particle) / Resolution: 5.3 Å

6729

5xlo

EMDB-6729, PDB-5xlo:
Anti-CRISPR proteins AcrF1/2 bound to Csy surveillance complex with a 32nt spacer crRNA backbone region
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-6730:
Anti-CRISPR protein AcrF1 bound to Csy surveillance complex with 32nt spacer crRNA in binding mode B
Method: EM (single particle) / Resolution: 4.7 Å

6731

5xlp

EMDB-6731, PDB-5xlp:
Anti-CRISPR proteins AcrF1/2 bound to Csy surveillance complex with a 20nt spacer crRNA backbone region
Method: EM (single particle) / Resolution: 4.2 Å

Source
  • pseudomonas aeruginosa (bacteria)
  • Pseudomonas phage D3 (virus)
  • Pseudomonas phage 73 (virus)
  • pseudomonas aeruginosa (strain ucbpp-pa14) (bacteria)
  • pseudomonas phage jbd30 (virus)
KeywordsIMMUNE SYSTEM/RNA / anti-CRISPR protein / Csy complex / Type I-F CRISPR/Cas system / IMMUNE SYSTEM-RNA complex / anti-CRISPR proteins

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