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Structure paper

TitleFast iodide-SAD phasing for high-throughput membrane protein structure determination.
Journal, issue, pagesSci Adv, Vol. 3, Page e1602952-e1602952, Year 2017
Publish dateApr 20, 2016 (structure data deposition date)
AuthorsMelnikov, I. / Polovinkin, V. / Kovalev, K. / Gushchin, I. / Shevtsov, M. / Shevchenko, V. / Mishin, A. / Alekseev, A. / Rodriguez-Valera, F. / Borshchevskiy, V. ...Melnikov, I. / Polovinkin, V. / Kovalev, K. / Gushchin, I. / Shevtsov, M. / Shevchenko, V. / Mishin, A. / Alekseev, A. / Rodriguez-Valera, F. / Borshchevskiy, V. / Cherezov, V. / Leonard, G.A. / Gordeliy, V. / Popov, A.
External linksSci Adv / PubMed:28508075
MethodsX-ray diffraction
Resolution2 - 2.8 Å
Structure data

PDB-5jgp:
Crystal structure of the nitrate/nitrite sensor NarQ fragment bound with iodide ions
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-5jrf:
Crystal structure of the light-driven sodium pump KR2 bound with iodide ions
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-5jsi:
Structure of membrane protein
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-5jtb:
Crystal structure of the chimeric protein of A2aAR-BRIL with bound iodide ions
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

Chemicals

ChemComp-NO3:
NITRATE ION

ChemComp-IOD:
IODIDE ION

ChemComp-HOH:
WATER

ChemComp-LFA:
EICOSANE

ChemComp-OLC:
(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

ChemComp-RET:
RETINAL

ChemComp-ZMA:
4-{2-[(7-amino-2-furan-2-yl[1,2,4]triazolo[1,5-a][1,3,5]triazin-5-yl)amino]ethyl}phenol / antagonist*YM

ChemComp-CLR:
CHOLESTEROL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-OLA:
OLEIC ACID

ChemComp-OLB:
(2S)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

Source
  • escherichia coli (strain k12) (bacteria)
  • dokdonia eikasta (bacteria)
  • candidatus actinomarina minuta (bacteria)
  • homo sapiens (human)
  • escherichia coli (E. coli)
KeywordsSIGNALING PROTEIN / membrane protein / sensor / histidine kinase / iodide / TRANSFERASE / TRANSPORT PROTEIN / sodium pump / UNKNOWN FUNCTION

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