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Structure paper

TitleRsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket.
Journal, issue, pagesNucleic Acids Res, Vol. 45, Issue 11, Page 6945-6959, Year 2017
Publish dateJun 20, 2017
AuthorsJorge Pedro López-Alonso / Tatsuya Kaminishi / Takeshi Kikuchi / Yuya Hirata / Idoia Iturrioz / Neha Dhimole / Andreas Schedlbauer / Yoichi Hase / Simon Goto / Daisuke Kurita / Akira Muto / Shu Zhou / Chieko Naoe / Deryck J Mills / David Gil-Carton / Chie Takemoto / Hyouta Himeno / Paola Fucini / Sean R Connell /
PubMed AbstractDuring 30S ribosomal subunit biogenesis, assembly factors are believed to prevent accumulation of misfolded intermediate states of low free energy that slowly convert into mature 30S subunits, ...During 30S ribosomal subunit biogenesis, assembly factors are believed to prevent accumulation of misfolded intermediate states of low free energy that slowly convert into mature 30S subunits, namely, kinetically trapped particles. Among the assembly factors, the circularly permuted GTPase, RsgA, plays a crucial role in the maturation of the 30S decoding center. Here, directed hydroxyl radical probing and single particle cryo-EM are employed to elucidate RsgA΄s mechanism of action. Our results show that RsgA destabilizes the 30S structure, including late binding r-proteins, providing a structural basis for avoiding kinetically trapped assembly intermediates. Moreover, RsgA exploits its distinct GTPase pocket and specific interactions with the 30S to coordinate GTPase activation with the maturation state of the 30S subunit. This coordination validates the architecture of the decoding center and facilitates the timely release of RsgA to control the progression of 30S biogenesis.
External linksNucleic Acids Res / PubMed:28482099 / PubMed Central
MethodsEM (single particle)
Resolution5.16 Å
Structure data

3661

5no2

EMDB-3661, PDB-5no2:
RsgA-GDPNP bound to the 30S ribosomal subunit (RsgA assembly intermediate)
Method: EM (single particle) / Resolution: 5.16 Å

3662

5no3

EMDB-3662, PDB-5no3:
RsgA-GDPNP bound to the 30S ribosomal subunit (RsgA assembly intermediate without uS3)
Method: EM (single particle) / Resolution: 5.16 Å

3663

5no4

EMDB-3663, PDB-5no4:
RsgA-GDPNP bound to the 30S ribosomal subunit (RsgA assembly intermediate with uS3)
Method: EM (single particle) / Resolution: 5.16 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM / 5'-Guanylyl imidodiphosphate

Source
  • Escherichia coli K12 (bacteria)
  • escherichia coli k-12 (bacteria)
  • escherichia coli (strain k12) (bacteria)
  • Escherichia coli (E. coli)
KeywordsRIBOSOME

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