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Structure paper

TitleCrystal Structure of the Minimal Cas9 from Campylobacter jejuni Reveals the Molecular Diversity in the CRISPR-Cas9 Systems
Journal, issue, pagesMol. Cell, Vol. 65, Page 1109-1121.e3, Year 2017
Publish dateJan 31, 2017 (structure data deposition date)
AuthorsYamada, M. / Watanabe, Y. / Gootenberg, J.S. / Hirano, H. / Ran, F.A. / Nakane, T. / Ishitani, R. / Zhang, F. / Nishimasu, H. / Nureki, O.
External linksMol. Cell / PubMed:28306506
MethodsX-ray diffraction
Resolution2.3 - 2.4 Å
Structure data

PDB-5x2g:
Crystal structure of Campylobacter jejuni Cas9 in complex with sgRNA and target DNA (AGAAACC PAM)
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-5x2h:
Crystal structure of Campylobacter jejuni Cas9 in complex with sgRNA and target DNA (AGAAACA PAM)
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

Source
  • campylobacter jejuni subsp. jejuni serotype o:2 (strain atcc 700819 / nctc 11168) (Campylobacter)
  • campylobacter jejuni (Campylobacter)
  • synthetic construct (others)
KeywordsHYDROLASE/RNA/DNA / CRISPR-Cas9 / RNA / DNA / Complex / Nuclease / HYDROLASE-RNA-DNA complex

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