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TitleA Versatile System for High-Throughput In Situ X-ray Screening and Data Collection of Soluble and Membrane-Protein Crystals.
Journal, issue, pagesCryst Growth Des, Vol. 16, Page 6318-6326, Year 2016
Publish dateJun 21, 2016 (structure data deposition date)
AuthorsBroecker, J. / Klingel, V. / Ou, W.L. / Balo, A.R. / Kissick, D.J. / Ogata, C.M. / Kuo, A. / Ernst, O.P.
External linksCryst Growth Des / PubMed:28261000
MethodsX-ray diffraction
Resolution1.7 - 2.125 Å
Structure data

PDB-5kkh:
2.1-Angstrom In situ Mylar structure of bacteriorhodopsin from Haloquadratum walsbyi (HwBR) at 100 K
Method: X-RAY DIFFRACTION / Resolution: 2.125 Å

PDB-5kki:
1.7-Angstrom in situ Mylar structure of hen egg-white lysozyme (HEWL) at 100 K
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-5kkj:
2.0-Angstrom In situ Mylar structure of hen egg-white lysozyme (HEWL) at 293 K
Method: X-RAY DIFFRACTION / Resolution: 2.001 Å

PDB-5kkk:
1.7-Angstrom In situ Mylar structure of sperm whale myoglobin (SWMb-CO) at 100 K
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-OLC:
(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

ChemComp-OLB:
(2S)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

ChemComp-RET:
RETINAL

ChemComp-HOH:
WATER

ChemComp-NA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-ACY:
ACETIC ACID

ChemComp-PE5:
3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL / precipitant*YM

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-CMO:
CARBON MONOXIDE

ChemComp-SO4:
SULFATE ION

Source
  • haloquadratum walsbyi (strain dsm 16790 / hbsq001) (archaea)
  • gallus gallus (chicken)
  • physeter catodon (sperm whale)
KeywordsMEMBRANE PROTEIN / TRANSPORT PROTEIN / HYDROLASE / OXYGEN TRANSPORT

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