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TitleDimerisation induced formation of the active site and the identification of three metal sites in EAL-phosphodiesterases.
Journal, issue, pagesSci Rep, Vol. 7, Page 42166-42166, Year 2017
Publish dateFeb 17, 2015 (structure data deposition date)
AuthorsBellini, D. / Horrell, S. / Hutchin, A. / Phippen, C.W. / Strange, R.W. / Cai, Y. / Wagner, A. / Webb, J.S. / Tews, I. / Walsh, M.A.
External linksSci Rep / PubMed:28186120
MethodsX-ray diffraction
Resolution1.6 - 2.44 Å
Structure data

PDB-4y9m:
PA3825-EAL Metal-Free-Apo Structure
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-5m1t:
PaMucR Phosphodiesterase, c-di-GMP complex
Method: X-RAY DIFFRACTION / Resolution: 2.27 Å

PDB-5mf5:
PA3825-EAL Mg-CdG Structure
Method: X-RAY DIFFRACTION / Resolution: 1.77 Å

PDB-5mfu:
PA3825-EAL Mn-pGpG Structure
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-5mkg:
PA3825-EAL Ca-CdG Structure
Method: X-RAY DIFFRACTION / Resolution: 2.44 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION

ChemComp-HOH:
WATER

ChemComp-C2E:
9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one)

ChemComp-MG:
Unknown entry

ChemComp-MN:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-CA:
Unknown entry

Source
  • pseudomonas aeruginosa pao1 (bacteria)
  • pseudomonas aeruginosa (bacteria)
  • synthetic construct (others)
KeywordsUNKNOWN FUNCTION / EAL / Cyclic di-GMP / SIGNALING PROTEIN / EAL domain Phosphodiesterase / HYDROLASE / Phosphodiesterase / biofilm formation / P Aeruginosa / PA3825

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