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Structure paper

TitleA beta-Mannanase with a Lysozyme-like Fold and a Novel Molecular Catalytic Mechanism.
Journal, issue, pagesACS Cent Sci, Vol. 2, Page 896-903, Year 2016
Publish dateMay 9, 2016 (structure data deposition date)
AuthorsJin, Y. / Petricevic, M. / John, A. / Raich, L. / Jenkins, H. / Portela De Souza, L. / Cuskin, F. / Gilbert, H.J. / Rovira, C. / Goddard-Borger, E.D. ...Jin, Y. / Petricevic, M. / John, A. / Raich, L. / Jenkins, H. / Portela De Souza, L. / Cuskin, F. / Gilbert, H.J. / Rovira, C. / Goddard-Borger, E.D. / Williams, S.J. / Davies, G.J.
External linksACS Cent Sci / PubMed:28058278
MethodsX-ray diffraction
Resolution0.96 - 1.18 Å
Structure data

PDB-5jts:
Structure of a beta-1,4-mannanase, SsGH134.
Method: X-RAY DIFFRACTION / Resolution: 1.09 Å

PDB-5ju9:
Structure of a beta-1,4-mannanase, SsGH134, in complex with Man3.
Method: X-RAY DIFFRACTION / Resolution: 1.18 Å

PDB-5jug:
Structure of an inactive (E45Q) variant of a beta-1,4-mannanase, SsGH134, in complex with Man5
Method: X-RAY DIFFRACTION / Resolution: 0.96 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-MAN:
alpha-D-mannopyranose

Source
  • streptomyces sp. nrrl b-244 (bacteria)
  • streptomyces sp. nrrl b-24361 (bacteria)
  • streptomyces sp. nrrl b-16215 (bacteria)
KeywordsHYDROLASE / beta-1 / 4-mannanase / carbohydrate degrading / glycosyl hydrolase

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